Studies on the Penicillinase Produced by a Streptomyces sp. (Part I). Optimal Conditions for the Penicillinase Production by Streptomyces sp. YS-40.

Streptomyces sp.가 생산하는 Penicillinase 에 관한 연구 (제1보) Streptomyces sp. YS-40에 의한 Penicillinase의 생산조건

Studies were carried out to investigate the optimal culture conditions for the production of penicillinase using a strain of Streptomyces sp. isolated from soil, YS-40. Among the carbon and nitrogen sources, glucose and L-asparagine increased the peniciilinase production. The addition of M $n^{＋＋}$, $Ca^{＋＋}$ and L $i^{＋}$ increased the enzyme production, but depressed by F $e^{＋＋＋}$, F $e^{＋＋}$, $Mg^{＋＋}$, Z $n^{＋＋}$, A $g^{＋＋}$, $Ba^{＋＋}$ and S $n^{＋＋}$. L-Leucine slightly increased the enzyme production but L-histidine, L-methionine depressed. Among the vitamins riboflavine, i-inositol, hesperidine, niacin-amide, biotin, folic acid, DL-$\alpha$-lipoic acid increased the enzyme formation. The addition of cephradine, cephalexin, ampicillin, cloxacillin more increased the enzyme formation than that of other$\beta$-lactam antibiotics and antibiotics. Optimal pH and temperature on the enzyme formation was pH 7.0 and 28$^{\circ}C$ respectively Amount of the enzyme production reached at maximum with incubation for 3 days on the optimal condition.

토양에서 분리, 선별한 Streptomyces 속의 한 균주가 생산하는 Penicillinase의 최적생산조건을 조사한 결과를 요약하면 다음과 같다. 탄소원으로서는 glucose, 실소원으로는 L-as-paragine이 가장 우수했으며 금속감중에서 Mn C $l_2$를 첨가했을때 효소생성을 37% 증가시켰다. Amino 산중에서 L-leucine이 본 효소생산을 약간 증가시켰으며 L-histidine L-methionine은 크게 저하시켰다. Riboflavine, i-inositol, hesperidine, niacinamide, biotin, DL-$\alpha$- lipoic acid, folic acid가 효소생산을 증가시켰으며 항생물질중에서 cephradine, cephalexin, ampicillin, cloxacillin의 첨가가 효소생성을 크게 증가시켰다. 본 효소생산의 최고 pH는 7.0이며 최적온도는 28$^{\circ}C$였다. 또 500$m\ell$ Erlenmeyer flask에서 175$m\ell$의 배지를 가하여 3 일간 배양했을 때 효소생성이 최고에 도달했다.