A Study on Immune Response by Intoxication of Parathion, Chloroform and 2,6-Dichlorophenol in Rats

Parathion, Chloroform 및 2,6-Dichlorophenol의 중독에 의한 흰쥐의 혈액학적 소견과 면양적혈구에 대한 항체 생산세포수에 미치는 영향

Among the environmental pollutants, parathion, chloroform and 2, 6-dichlorophenol may impair human health; they may inhibit or reduce the metabolic function of human body and may furthermore cause diseases directly or indirectly. This study was undertaken to investigate the effects on the immune response by intoxication of parathion, chloroform or 2, 6-dichlorophenol. Parathion(1.3mg/kg, olive oil 10ml), chloroform (100mg/kg, olive oil 10ml) were administered via intraperitoneal injection to rats. And 2, 6-dichlorophenol (13mg/kg, olive oil 10ml) was administered via oral injection. After 3 weeks, the rats were intoxicated with the above chemicals and immunized with sheep RBC. After 4 weeks the immune response of rat spleen cells was measured by the Jerne's technique. The results were obtained as follows. 1. There was no change of leukocyte counts by the intoxication of parathion, chloroform and 2, 6-dichlorophenol. 2. Parathion, chloroform and 2, 6-dichlorophenol reduced hemoglobin contents for most intoxicated and immunized groups. 3. Hematocrits were decreased by the intoxication of parathion, chloroform or 2, 6-dichlorophenol significantly. 4. It was determined that total protein, A/G (albumin/globulin), .alpha.-, .betha.-and .gamma.-globulins in rat serum were not changed. 5. Intoxication by parathion, chloroform or 2, 6-dichorophenol reduced the number of hemolytic plaque to the sheep RBC in rat spleen cells. Therefore, the capacity of erythrocyte production and the immune response of rat spleen cells were decreased by the intoxication of parathion, chloroform, or 2, 6-dichlorophenol.