불포화지방산(不飽和脂肪酸)의 비색정량법(比色定量法)

Colorimetric Method of Determining Unsaturated Fatty Acids in Animal Tissues

  • 허린수 (경북대학교 농과대학 수의학과) ;
  • 장인호 (경북대학교 농과대학 수의학과)
  • Huh,, Rhin-Sou (Dept. of Vaterinary Medicine, College of Agriculture Kyungpook National University) ;
  • Chang, In-Ho (Dept. of Vaterinary Medicine, College of Agriculture Kyungpook National University)
  • 발행 : 1982.03.30

초록

The present paper describes a colorimetric method of determining the free and total unsaturated fatty acids in animal tissues. The procedure is based in three steps on the following principles : First step is that the tissue homogenates are extracted in chilled acetone in order to eliminate the interfering substance, such as phospholipids, sulfatides and proteins. At next step, after centrifugation acetone layer is decanted and evaporated to dryness. Then the extract is shaken with heptane to solve in the solvent. That the characteristic nature of copper salts of unsaturated fatty acids are freely soluble in heptane and those of saturated acids are not is the bases of separating one from another. Thus unsaturated fatty acids can be isolated in heptane as their salts from saturated acids and other lipid mixture by shaking with copper reagent. Finally the yellowish brown color developed by adding color reagent (0.2% sodium diethyldithiocarbamate in n-butanol solution) which reacts with the copper salts of the acids and produces the color is measured colorimetrically.

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