Studies on the Processing Adaptability of Hazel Nut - Separation of Protein from Defatted Hazel Nut Meal and Characterization of the Protein Isolates -

개암종실(種實)의 가공적성(加工適性)에 관(關)한 연구(硏究) - 개암종실(種實) 탈지박(脫脂粕)의 단백질(蛋白質) 분리(分離) 및 분리단백질(分離蛋白質)의 특성(特性)에 대하여

In order to obtain the basical data for utilization of protein in hazel nut, protein was extracted from defatted hazel nut meal with salt solutions and alkaline solutions, and precipitated by adjusting pH of extract to 5.5 or addition of organic solvents. Amino acid composition of the isolated protein and defatted hazel nut meal were analyzed, protein isolates were identified by polyacrylamide gel electrophoresis. The results summarized were as follows. 1. Defatted hazel nut showed highly nutritional value as the content of 'protein was 53.6%. 2. Extractabilities of salt-soluble protein treated with 2.5M $MgCl_2$, and 1M NaCl(pH 11.0) were 53.0%, 31.5%, respectively 3. Protein in hazel nut were contained 53% of salt-soluble globulin, 14% of water-soluble albumin, 29.5% of glutelin based on solubility. 4. At pH 5.5, 85% of the extracted protein was precipitated, and about 90% of the extracted protein was separated by addition of organic solvents such as acetone and ethanol at 60-70% concentration. 5. Proteins extracted from defatted hazel nut with water and 0.027N NaOH showed 3 and 6 hands by polyacrylamide gel electrophoresis, respectively. 6. Amino acids of defatted hazel nut and protein isolate were chiefly composed of glutamic acid, arginine and aspartic acid.

개암종실(種實)중의 단백질(蛋白質)을 이용하기 위한 기초자료을 억디 위하여 탈지박(脫脂粕)으로부터 단백질(蛋白質)을 추출(抽出)하여 침전(沈澱)으로 분리(分離)하고 분리단백질(分離蛋白質)의 아미노산 조성(組成)과 전기영동(電氣泳動) pattern을 검토한 결과는 다음과 같다. l. 탈지박(脫脂粕)의 단백질함량(蛋白質含量)은 54.6%로서 높은 영양가가 인정되었다. 2. 탈지박(脫脂粕)의 염용해성단백질의 추출율(抽出率)은 2.5M $MgCl_2$에 있어서 53.0%, 1.0M NaCl에 있어서 31.5%이었다. 3. 개암단백질(蛋白質)은 용해도(溶解度)에 따라 염용해성 globulin 53.0%, 수용성(水溶性)albumin 14.0%, glutelin 29.5%로 분류(分類)되어졌다. 4. 추출(抽出)된 단백질(蛋白質)의 침전율(沈澱率)은 pH5.5에서 85%이었으며 ethanol과 acetone의 농도를 60~70%로 하였을 때 90%의 단백질이 분리(分離)되어졌다. 5. 전기영동(電氣泳動)에 의하여 물과 0.01M NaCl로 추출된 단백질(蛋白質)은 3개의 band, 0.027N NaOH와 1M NaCl에 있어서는 6개의 band가 나타났다. 6. 개암종실탈지박(種實脫脂粕)과 분리단백질(分離蛋白質)이 주된 아미노산은 glutamicacid, arginine, aspartic acid이었다.