The Korean Journal of Nuclear Medicine (대한핵의학회지)

The Korean Society of Nuclear Medicine (대한핵의학회)
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A study on Radiolabelling Method in Radioimmunoassy -Part 2. Determination of Serum Thyrotropin using new Radiolabelling Method-

방사면역측정(放射免疫測定)에서 방사성동위원소(放射性同位元素) 표식법(標識法) 개발에 관한 연구(硏究) -제2편(第2編) 새로운 표식법(漂識法)을 이용한 혈청(血淸) 갑상선자극(甲狀腺刺戟) 홀몬 측정(測定)-

  • Cho, Bo-Yeon (Department of Internal Medicine, College of Medicine, Seoul National University) ;
  • Suh, Jung-Hun (Depart. of Chemistry, Seoul National University) ;
  • Park, Yong-Ja (Depart. of Nuclear Medicine, Seoul National Univ. Hosp.) ;
  • Kim, Byung-Kook (Department of Internal Medicine, College of Medicine, Seoul National University) ;
  • Koh, Chang-Soon (Department of Internal Medicine, College of Medicine, Seoul National University)
  • 조보연 (서울대학교 의과대학 내과학교실) ;
  • 서정헌 (서울대학교 화학과) ;
  • 박영자 (서울대학교 핵의학과) ;
  • 김병국 (서울대학교 의과대학 내과학교실) ;
  • 고창순 (서울대학교 의과대학 내과학교실)
  • Published : 1981.11.25
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Abstract

The radioimmunoassay of human thyrtropin was performed in various thyroid diseases, using the antin-h-TSH antibody (Daiichi, Japan) and purified h-TSH(Biodata, Italy). $^{125}I$ labelling of h-TSH was performed using a small amount $(1.8{\mu}g)$ of chloramin-T as an oxidant at room temperature. This new method facilitated uniform labelling and reduced to damage of h-TSH by chloramin-T, and satisfactory radioimmunoasasy results were obtained with $^{125}I-TSH$ prepared by the new method until at least 7 weeks after preparation, The assay sensitivity was $1.0{\mu}u/ml$. The coefficient of variances in within assay and interrun assay were all less than 10% among the range of $1.25{\mu}u/ml$ and $160{\mu}u/ml$. The serum TSH values by this new method were colsely related to those by commercial kit (r= 0.996).

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