The Effect of synthetic Antioxidants on the Proteolytic enymes 1. The Effect of synthetic Antioxidants on the Activity of the $\alpha$-Chymotrypsin and Trypsin

합성 항산화제가 단백질 분해효소에 미치는 영양 -제1보,$\alpha$ -Chymotrypsin 과 trypsin의 활성에 미치는 영양-

This study was conducted to investigate the effects of synthetic antioxidants con the degradation of angiotensin II which is made up of 8 amino acids: Asp-Arg-Val-Try-Ile-Gly-Pro-Phe, by the $\alpha$-chymotrypsin and trypsin. the results obtained were as follow; 1. Dibutyl hydroxytoluene, butyl hydroxyanisole and sodium L-ascorbate showed no inhibitory effect on the activity of $\alpha$-chymotrypsin on the angiotensin II, but ethyl protocathechuate inhibited. its activity at the concentration of 100ppm. However, the angiotension II was gradually degradated by $\alpha$-chymotrypsin after one hour incubation with ethylprotecathechuate. 2. Butyl hydroxyanisole inhibited trypsin activities above 100ppm, but no inhibitory activities was observed by the other antioxidants used in this experiment.