Restorative Dentistry and Endodontics

The Korean Academy of Conservative Dentistry (대한치과보존학회)
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ISOLATION AND BIOTYPING OF STREPTOCOCCUS MUTANS FROM DENTAL PLAQUE OF CARIOUS LESION

충치나환부(蟲齒羅患部) 치태(齒苔)로 부터 Streptococcus mutans의 분리(分離)·동정(同定)과 biotyping에 대한 연구(硏究)

  • Park, Sang-Jin (Department of Microbiology, School of Medicine, Kyung Hee University) ;
  • Lee, Jean-Yong (Department of Microbiology, School of Medicine, Kyung Hee University) ;
  • Chung, Choong-Mo (Department of Operative Dentistry, School of Dentistry, Kyung Hee University) ;
  • Ha, Youn-Mun (Department of Operative Dentistry, School of Dentistry, Kyung Hee University) ;
  • Choi, Ho-Young (Department of Operative Dentistry, School of Dentistry, Kyung Hee University)
  • 이진용 (경희대학교 치과대학 미생물학교실) ;
  • 하윤문 (경희대학교 치과대학 미생물학교실) ;
  • 정충모 (경희대학교 치과대학 보존학교실) ;
  • 박상진 (경희대학교 치과대학 보존학교실) ;
  • 최호영 (경희대학교 치과대학 보존학교실)
  • Published : 1980.12.31
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Abstract

Streptococcus mutans were isolated from dental plaques of carious lesions of 4 patients on mitis-salivarius agar medium. Three patients known to harbor S. mutans in their dental plaques. Identification of the isolated S. mutans was established by colonial morphology on mitis-salivarius agar medium, the fermentation of mannitol and sorbitol, and confirmed by agglutinating reaction with home made anti-S. mutans NCTC 10449 (serotype c) antiserum. Of the isolated S. mutans, one strain (P2-1) showed strong agglutinating reaction with antiserum, another strain (P1-2) showed weak agglutinating reaction. P2-1 strongly adhered to the wall of the test tube containing 5% sucrose broth, while p1-2 weakly colonized on the wall of the test tube. Biotyping of the isolated S. mutans based on the fermentation of mannitol, sorbitol, raffinose and melibiose, and the production of ammonia from L-arginine, and the inhibition of acid production by bacitracin. Biochemical characteristics of P2-1 strain correlated with the recognized biotype c, pl-2 strain resembled biotype d of S. mutans.

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