Treatment of E. coli B with two Antibiotics and their Influence on $T_3$ phage Absorption

대장균(E. coli B)의 항생제처리가 $T_3$ phage의 부착에 미치는 영향

E. coli B was treated with colistin and kanamycin and the influence of these antibiotics on the absorption of $T_3$ phage was studied using the plaque counting method and the electron microscope. E. coli B treated with colistin was sharply inhibited on phage absorption and cell walls were severely damaged showing some spiny appearance around the walls. No influence of kanamycin was noted on phage absorption. Bacterial cells treated with kanamycin showed wave form in the structure of walls and a profound change was noted in the cytoplasm where it was concentrated along the periphery of the inner wall leaving the center of the cell to appear almost empty.