Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Larqe guantity isolation of Ginsenoside $-Rb_1,\;-Rb_2,\;-Rc,\;-Rd,\;-Re\;and\;-Rg_1$ in Panax ginseng C.A. Meyer by High Performance Liquid Chromatography

고속액체(高速液體) chromatography에 의(依)한 Ginsenoside $-Rb_1,\;-Rb_2,\;-Rc,\;-Rd,\;-Re$ 및 $-Rg_1$의 대량분리(大量分離)

  • Choi, Jin-Ho (Korea Ginseng Research Institute) ;
  • Kim, Woo-Jung (Korea Ginseng Research Institute) ;
  • Bae, Hyo-Won (Korea Ginseng Research Institute) ;
  • Oh, Sung-Ki (Department of Food Technology, Kyung Hee University) ;
  • Oura, Hikokichi (Department of Biochemistry, Research Institute for WAKAN-YAKU, Toyama Medical and Pharmaceutical University)
  • Published : 1980.12.31
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Abstract

Relatively large quantity of the major components of saponin, $ginsenoside-Rb_1,\;-Rb_2,\;-Rc,\;-Rd,\;-Re\;and\;-Rg_1$ from Panax ginseng C.A. Meyer were isolated using preparative and semipreparative high performance liquid chromatography, and analyzed by analytical HPLC. The application of HPLC for isolation of ginsenosides was not only very effective for rapid analysis but also reduced the isolation time. The isolation capacity of pure ginsenosides was $30{\sim}50mg/hr$.

인삼의 유효약리성분으로 밝혀진 saponin중의 각 ginsenosides를 효과적이고 능률적으로 분리하기 위하여 대량분취전용 고속액체 chromatograph인 preparative HPLC의 응용을 검토하였다. 조(粗) saponin획분을 preparative HPLC인 Prep LC/system-500를 사용하여 부분분획을 하고 각 획분에 함유되어 있는 ginsenosides의 조성을 Analytical HPLC로 동정한 후 Semi-preparative HPLC를 사용하여 인삼주성분 saponin을 단리했다. 그 결과 인삼 주성분 saponin인 $ginsenoside-Rb_1,\;-Rb_2,\;-Rc,\;-Rd,\;-Re$ 및 $-Rg_1$은 약 20 mg / 2.0 ml / injection으로 chromatography를 행하여 $300{\sim}400mg/day$로 대량분취가 가능하였다. 따라서 ginsenosides의 약리 및 임상효능 연구에 크게 기여하게 될 것이다.

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