Autofluorescence-elicitor activity of ethanol-extract fraction from conidia of Erysiphe graminis hordei to the leaf of barley

흰가루병균 분생포자 ethanol 추출분획의 대맥엽 형광화세포 유도활성

The autofluorescent cells in the penetration area of powdery mildewed leaves of barley had been reported. The present experiments were performed in order to obtain the fluorescence-inducing extract fraction from the conidia. The preparations were made by extractions and residue which were extracted from the conidia of Erysiphe graminis hordei race I with water, ethanol, and ethyl ether. Bioassaying was carried out on the culled-leaf surface of incompatible Turkey 290 and compatible Kobingataki varieties by placing the drops of extract solutions. Fluorescence-elicitor activity was shown only in tile ethanol-extract fraction to both varieties. However, fluorescence-eliciting rate was more rapid on the leaves of incompatible variety Turkey 290 than on tile those of compatible variety Kobingataki; Turkey 290 less than 8 hours, Kobingataki less than 16 hours.

보리흰가루병(Erysiphe graminis hordei race I)에 의한 대맥엽침입부에 항균성 형광화세포를 유도하는 물질의 추출분획을 얻기 위하여 실험을 실시한 결과 분생포자의 ethanol 추출분획이 형광화세포의 유도활성을 가지고 있음이 밝혀졌다. 이 분획은 비친화성인 Turkey 290품종에나 친화성품종에나 마찬가지로 형광화세포 유도활성을 나타냈으나 그 유도에 요하는 시간은 Turkey 290에서 8시간 이내 Kobingataki에서 16시간이내이었다.