Abstract
1. The study was conducted on the optimum condition of the treated substrate with dilute sulphuric acid solution and cellulase for saccharification. The wood (saw dust) of Alnus hirsuta Rupr. (10~15 years) was treated with 0.3%, 0.6%, 0.9%, 1.2%, 1.5%, $H_2SO_4$ solution at $1.5kg/cm^2$ for 15min., 30min., 45min., and 60min., followed by thermal treatment at $190^{\circ}C$ for 30min., and screening with 60 mesh sieve, after which to 0.5 grams of each sample was added 0.5ml cellulase solution, and 50ml 0.1M acetic acid buffer solution (pH 5.0), after incubating at $40^{\circ}C$ for 96hr. 2. The crude cellulase of Trichoderma viride Perx. ex. Fr. SANK 16374 was produced by the submerged culture process and produced in the culture fluid was salted out quantitatively by the use of ammonium sulfite. 3. Reducing sugar was determined by the 3.5-dinitrosalicylic acid (DNS) method. 4. The reducing sugar was increased with increase of the sulphuric acid concentration and saw dust was treated with 1.5% $H_2SO_4$ solution at $1.5kg/cm^2$ for 45min. showed the best saccharification (16.0%). The reducing sugar formation did not show statistically significant in 5% levels by thermal treatment time 45min. and 60min. 5. The substrate for cellulase which was treated with 0.9% $H_2SO_4$ solution at $1.5kg/cm^2$ for 60min. showed the best reducing sugar formation (23.6%). And did not show significant difference in 5% levels at 0.9%, 1.2%, and 1.5% $H_2SO_4$ solution.
1. 본시험(本試驗)은 당화기질(糖化基質)로 산오리나무재(材)를 묽은황산(黃酸)으로 전처리(前處理)한 후(後) Trichoderma viride 16374호균(號菌)에서 얻은 cellulase를 작용(作用)시켜 환원당(還元糖)을 생성(生成)할 수 있는 최적조건(最適條件)을 조사(調査)한 것이다. 즉(卽) 산오리나무(10~15년생(年生))의 톱밥을 전처리(前處理)할 때 황산농도(黃酸濃度) 0.3%, 0.6%, 0.9%, 1.2%, 1.5%로 구분(區分)하고 열압시간(熱壓時間)은 $1.5kg/cm^2$에서 15분(分), 30분(分), 45분(分), 60분(分)으로 각각(各各) 구분(區分) 처리(處理)하여 건조(乾燥)시킨것을 다시 $190^{\circ}C$에서 30분간(分間) 열처리후(熱處理後) 60mesh로 분쇄(粉碎)한 것을 당화기질(糖化基質)로 사용(使用)하였다. 그리고 cellulase 반응조건(反應條件)은 0.1M황산완충액(黃酸緩衝液)(pH 5.0) 50ml, 기질량(基質量) 0.5g, 고근효소액(盬析酵素液) 0.5ml를 100ml용(用) 삼각(三角) flask에 넣고 잘 혼합(混合)한 후(後) $40^{\circ}C$에서 96시간(時間) 반응(反應)시켜 이때 생성(生成)된 환원당량(還元糖量)을 조사(調査)하였다. 2. 고근효소액(盬析酵素液)의 조제(調製)는 밀기울액체진탕배양법(液體振盪培養法)에 의(依에) 하여 생성(生成)된 조효소액(粗酵素液)을 유안절포도(硫安飽和度)에 의(依)한 고근효소액(盬析酵素液)을 만들었다. 3. 환원당정량(還元糖定量)은 DNS법(法)에 의(依)하였다. 4. 묽은황산(黃酸)으로 처리(處理)한 톱밥의 환원당(還元糖) 생성량(生成量)은 황산농도(黃酸濃度) 1.5%, $1.5kg/cm^2$에서 열압시간(熱壓時間)은 45분(分)으로 처리(處理)한 것이 16.0%로 최대량(最大量)을 나타냈으나 작용(作用)함으로서 생성(生成)된 환원당량(還元糖量)은 황산농도(黃酸濃度) 0.9%, 열압시간(熱壓時間) 60분(分) 처리(處理)한 것이 23.6%로(본시험(本試驗) 범위(範圍)에서는 47.5% 증가(增加)) 나타났다.