Serological Detection of Plant Viruses with latex-test

Latex-Test에 의한 식물 바이러스의 검정

A simple test using latex-agglutination was developed to detect serologically tobacco mosaic virus (TMV), soybean mosaic virus (SoyMV), and barley stripe mosaic virus (BSMV) from infected Plants. Latex spheres ( 0.81 $\mu$, Difco) were adsorbed with immuno globulin purified by electrophoresis from crude antiserum against viruses. The antibody- sensitized latex suspension was mixed with sap from virus -infected leaves in a glass capillary tube (inner diam. 1mm $\times$ 100 mm length) The mixture, after agitation, was observed under a stereo microscope at low magnification (X20 - X4O), to examine the reaction between antigen (Virus) and its antibody. Flocculation occurred when the reaction was positive.

低濃度의 바이러스 조건하에서도 반응의 민감도가 높은 바이러스病의 血淸學的 診斷法을 개발하고자 담배모자이크 바이러스(Tobacco mosaic virus), 보리줄무늬 모자이크 바이러스( Barleys-tripe mosaic virus) 및 大豆모자이크 바이러스(Soybean mosaic virus)의 抗血淸으로부터 電氣泳動에 의해 추출된 Immunoglobulin을 Latex(0.8l$\mu$, Difco)粒子에 흡착시켜 梢子毛細管(1 $\times$ 100mm)내에서 各 罹病組織의 汁液과 반응시킨 결과 寒天沈降法이나 微量沈降法보다 민감도가 매우 높았다. 이 방법은 작업과정이 간편하고 빠른 시간내에 다량의 시료를 檢定할 수 있으며 陽性反應과 陰性反應을 쉽게 구별할 수 있었다.