Activities of Several Hydrolytic Enzymes in the Pear Leaves Affected by Rust Fungus, Gymnosporangium haraeanum Sydow

배나무적성병 병반조직의 몇가지 가수분해효소의 활성

Activities of several hydrolytic enzymes in the rusted pear loaves were estimated. Tested enzymes were cellulase (Cx), invertase $\beta-amylase$, pectinase (pectinmethylesterase and polygalacturonase), and phosphatase. Enzyme activities represented by pH value-enzyme activitycurve. Activities of Cx. invertase. and $\beta-amylase$ were higher in the healthy part than those in the near-lesion and lesion parts. and showed the maximal activities around pH 7.0. On the other hand, activities of pectinase and phosphatase were higher in the lesion and near-lesion parts than in the healthy part. Pectinmethylesterase activities in the healthy and diseased parts were different with different curve-pattern as the pH of treating solution changed.

적성병균에 감염된 배나무 잎에서 건전부위, 병반인접부위 및 병반부위별로 몇가지 가수분해효소의 활성을 측정한 결과는 다음과 같다. 1. Cellulase (Cx), Invertase 및 $\beta-amylase$의 활성은 감염부위에서 보다 건전부위에서 더 높았고 모두 pH 7.0부근인 중성측에서 최대활성이 나타났다. 2. Pectinmethylesterase, Polygalacturonase 및 Phosphatase의 활성은 병반인접부위에서 높았다. 3. Pectinmethylesterase의 활성은 건전부위와 병반 및 그인접부위 간에 pH에 따라 심한 활성차리가 인정되었다.