한국수산과학회지 (Korean Journal of Fisheries and Aquatic Sciences)
- 제11권2호
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- Pages.85-90
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- 1978
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- 0374-8111(pISSN)
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- 2287-8815(eISSN)
해조단백질 추출에 관한 연구 3. NaOH 가용성 단백질의 추출
STUDIES ON THE EXTRACTION OF SEA WEED PROTEINS 3. Extraction of NaOH Soluble Proteins
- LEE Kang-Ho (National Fisheries University of Busan) ;
- WOO Soon-Im (Seoul Women's University) ;
- RYU Hong-Soo (National Fisheries University of Busan)
- 발행 : 1978.06.01
초록
식용해조인 둥근돌김(Perphyra suborbiculata), 잎파래 (Enteromorpha linza), 구멍갈파래 (Ulva pertusa) 미역 (Undaria pinnatifida, 자연생 및 양식), 모자반 (Sargassum fulvellum), 쎌만모자반(Sargassum kjellmanianum) 누운첨각(Codium coarctatum)의 NaOH 가용성 단백질 추출에 영향을 미치는 제요소 즉, 추출용액의 농도, 용매량, 온도, 시간에 따른 조단백질과 순단백질(TCA 불용성 단백질)의 추출 결과를 요약하면 다음과 갈다. 1. 전시료가 0.05M NaOH 용액에서 가장 좋은 추출성적을 얻었으나, TCA 불용성 단백질은
In present study, the effect of various factors including the solvent concentration, extraction time, extraction temperature and the ratio of sample vs extraction solvent(w/v) upon the extractability of the NaOH soluble proteins of marine algae were investigated. Seven species of sun-dried algae, the major ones in consumption as food, namely Porphyra suborbiculata, Undaria pinnatifida(natural and cultivated), Sargassum fulvellum, Sargassum kjellmanianum, Ulva pertusa, Enteromorpha linza and Codium coarctatum were used for the extraction of the NaOH soluble protein. The frozen and masceratd samples were prepared by the same mettled described in previous paper(Lee, 1977). In case of the TCA insoluble protein, all samples reached maxima at 0.025M NaOH solution while the 0.05M for extractable total nitrogen. Variation of the ratio of sample vs solvent gave slight effect upon the extractability, 100 ml solvent added to 1 g dried sample was effective. The effect of extraction time on the extractability differed from species. The extractabilty of Enteromorpha linza, Ulva pertusa and Codium coarctatum reached maxima within 1 hour extraction and 2 hours for the cultivated Undaria pinnatifida while 3 flours for the natural Undaria pinnatifida, Sargassum fulvellum, Sargassum kjellmanianum and Porphyra suborbiculata. The most effective extraction temperature was
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