사람 글로빈의 Chain Anomaly 결정에 관한 연구

Studies on the Chain Anomaly Determination of Human Globins

  • 노일협 (숙명여자대학교 약학대학) ;
  • 최정숙 (숙명여자대학교 약학대학)
  • 발행 : 1978.12.01

초록

We studied on the separation of $\alpha$- and $\beta$-chain globins, the conditions of paper electrophoresis and mobility of globins in paper electrophoresis to determine their chain anomaly. Acidic-acetone mothod of Wilson and Smith was found to be more effecient for separating $\alpha$- and $\beta$chain globions than trichloroacetic acid method of Hayashi. The conditions of paper electrophoresis were current of 20v/cm and 0.6mA/cm for three hours at room temperature. In paper electroporesis, the mobility of $\alpha$- -chain globin towards the cathode was greater than that of $\beta$-chain globin. The results showed tha tchain anomaly of $\alpha$- and $\beta$-chain globins can be determined by the method of paper electrophoresis.

키워드

참고문헌

  1. J. Am. Chem. Soc. v.79 H.S.Rhinesmith;W.A.Schroeder;L.Paulng
  2. Z. Physiol. Chem. v.325 G.Braunizer;R. Gehring Muller;N.Hilschmann;K.Hilse;G.Hobom;V.Rudloff;B. Wittmann Liebold
  3. 淑明女子大學校 論文集 v.7 魯一協
  4. Science v.158 I.H.Ro(et al.)
  5. Am. J. Phys. Anthrop. I.H.Ro(et al.)
  6. 韓國營養學會誌 v.3 魯一協
  7. Blood v.24 A.I.Chernoff;N.M.Pettit
  8. J. Biochem. v.49 T.Take
  9. Adv. Clin. Chem. v.6 T.H.J.Huisman
  10. J. Gen. Physiol. v.13 M.L.Anson;A.F.Mirsky
  11. Can. J. Biochem. Physiol. v.37 S.Wilson;B.D.Smith
  12. J. Biochem. v.50 H.Hayashi
  13. Nature v.186 C.J.Muller