Studies on the Chain Anomaly Determination of Human Globins

사람 글로빈의 Chain Anomaly 결정에 관한 연구

  • 노일협 (숙명여자대학교 약학대학) ;
  • 최정숙 (숙명여자대학교 약학대학)
  • Published : 1978.12.01

Abstract

We studied on the separation of $\alpha$- and $\beta$-chain globins, the conditions of paper electrophoresis and mobility of globins in paper electrophoresis to determine their chain anomaly. Acidic-acetone mothod of Wilson and Smith was found to be more effecient for separating $\alpha$- and $\beta$chain globions than trichloroacetic acid method of Hayashi. The conditions of paper electrophoresis were current of 20v/cm and 0.6mA/cm for three hours at room temperature. In paper electroporesis, the mobility of $\alpha$- -chain globin towards the cathode was greater than that of $\beta$-chain globin. The results showed tha tchain anomaly of $\alpha$- and $\beta$-chain globins can be determined by the method of paper electrophoresis.

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