The Korean Journal of Physiology
- 제12권1_2호
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- Pages.15-23
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- 1978
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- 0300-4015(pISSN)
적혈구막의 NaK ATPase의 활성도에 대한 ascorbic acid의 작용
Action of Ascorbic acid on Sodium-Potassium activated ATPase in Red Cell Membrane
- 고일섭 (경희대학교 의과대학 생리학교실)
- Koh, Il-Sup (Department of Physiology, School of Medicine, Kyung Hee University)
- 발행 : 1978.10.31
초록
The action of ascorbic acid on the sodium Plus potassium activated ATPase activity in the rabbit red cell membrane has been investigated and the experiments were also designed to determine the mechanism of action if ascorbic acid on the ATPase activity The following results were observed. 1. The activity of the NaK ATPase from red cell membrane is stimulated by ascorbic acid and the concentration of ascorbic acid for maximal activity is about 8 mM. 2. The activating effect of ascorbic acid on the ATPase activaty, with a given concentration of sodium in the medium, is increased by raisins the potassium concentration but activity ratio is decreased. 3. The activating effect of ascorbic acid on the ATPase activity, with a given concentration of potassium in the medium, is increased by raising the sodium concentration but activity ratio is decreased. 4. The action of ascorbic acid on the ATPase activity is stimulated by calcium ions and activity ratio is increased by raising the calcium concentration. 5. The activating effect of ascorbic acid on the ATPase activity was not related to the sulfhydryl group of cysteine or the hydroxyl group of threonine. 6. The activating effect of ascorbic acid on the ATPase activity is due to amino group and carboxyl group of the enzyme of NaK ATPase.
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