Studies on the Chemical Resistance of Phytopathogenic Bacteria III. Some Variations on the Streptomycin Resistant Isolates of the Rice Bacterial Leaf Blight Pathogen, Xanthomonas oryzae(Uyeda et Ishiyama) Dowson

식물병원성세균의 약제저항성에 관한 연구 III. 벼 흰빛잎마름병균에 있어서 Streptomycin 내성균주의 몇 가지 변이

The experiments were contucted to investigate the variability of pathogenesity, growth amount and UV-sensitibity on the streptomycin resistant isolate of the rice bacterial leaf blight pathogen, Xanthomonas oryzae, which selected by the stepwise transfer in 100, 3,000 and 10,000 ug/ml Argepto contained media. And the results obtained were as follows. (1) The SM-resistant isolates were tested the pathogenesity on the differential variety of rice, wase-Aikoku-3, Rant Emas-2, Hwang ok, and Kimmase. And the SM resistant isolate, obtained from 75-6 isolate, showed the reaction of moderately resistant to the differential variety of Hwang ok instead of susceptible reaction with its parental isolate. (2) The growth amount of the SM-resistant isolate was slightly higher than that of parental isolate, on the normal media. And the growth was inhibited on the 100ug/ml Agrepto contained media until 60 hours after transfer, however, its growth exceeded than parental isolate in the normal media, after 70 hours of transfer. (3) It is considered that the resistance factor might be stable character, since the sensitivity to UV irradiation (with 254 mr wave length) of the resistant isolate was the same as that of its parental isolate.

선발에 의하여 얻은 벼 흰빛잎마름병균 Xanthomonas oryzae의 Streptomycin 내성균에 대하여 병원성, 배지내에서의 생장량 및 자외선 감수성에 대한 변이성을 조사하여 다음과 같은 결과를 얻었다. (1) SM 내성균은 Wase-Aikoku-3, Rant Emas-2 황옥 및 Kimmase의 4개 판별품종에 대한 병원성 비교에서 75-6의 내성균주가 황옥에 대하여 감수성 반응이 모균과 달리 중정도의 저항성을 나타내었다. 2) SM-내성균은 정상배지에서 모균보다 약간 높은 생장량을 보였고 100ug/ml Agrepto 첨가 배지에서는 접종후 60시간까지 생장이 조지되었으나 70시간 이후에는 모균의 생장량을 능가하였다. (3) SM-내성균은 254mu 파장의 자외선 조사에서 모균과 동일한 감수성을 나타내어 내성인자는 안정된 것으로 생각되었다.