Korean journal of applied entomology (한국응용곤충학회지)

Korean Society of Applied Entomology (한국응용곤충학회)
권호 표지

Purification and Serology of Potato Virus S

감자 바이러스 S의 순화와 항혈청제조

  • Lee Soon Hyung (Department of Plant Pathology, Institute of Agricultural Sciences, Office of Rural Development) ;
  • Lee Key Woon (Department of Plant Pathology, Institute of Agricultural Sciences, Office of Rural Development) ;
  • Chung Bong Jo (Department of Plant Pathology, Institute of Agricultural Sciences, Office of Rural Development)
  • 이순형 (농촌진흥청 농업기술연구소 식물병리과) ;
  • 이기운 (농촌진흥청 농업기술연구소 식물병리과) ;
  • 정봉조 (농촌진흥청 농업기술연구소 식물병리과)
  • Published : 1977.09.01
Download PDF
⟨ Previous Next ⟩

Abstract

he study was conducted to produce an antiserum of potato virus S for identification and screening of seed-potatoes. Potato virus S was isolated from infected plants and identified by means of indicator plants and electro microscopy. Isolated potato virus S was multiplied in Nicotiana deebneyii and the virus was purified by a modified method that was developed through this study. The purity of potato virus S was 1.18mg/ml. Purified potato virus S was injected into rabbit intravenously once a week for 5 weeks. Antiserum was collected 10 days after the last injection. The produced antiserum was determined to have a titer of, 1/2048 by means of microprecipitin tests.

감자바이러스 S(PVS)의 진단, 동정 및 씨감자의 검정에 이용할 항혈청을 만들기 위하여 이병주로부터 PVS를 순수분리 순화하여 항혈청을 제조하였다. PVS는 지표식물파 전자 현미경으로 순수 분리하여 Nicotiana debneyii에서 증식하여 순화하였다. 순화된 PVS의 순화도는 1.18mg/ml이었으며 이것을 1.5ml씩 7일 간격으로 5회 .토끼에 주사하였으며 마지막 주사후 10일에 채혈하여 항혈청을 분리하였다. 제조된 PVS항혈청의 역가는 미량침강법에 의하여 1/2048로 나타났다.

Keywords