Korean journal of applied entomology (한국응용곤충학회지)

Korean Society of Applied Entomology (한국응용곤충학회)
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Studies on Purification and Serology of Potato Virus X

감자바이러스 X의 순화와 혈청학적 연구

  • Lee Soon Hyung (Department of Plant Pathology, Institute of Agricultural Sciences, Office of Rural Development) ;
  • Lee Key Woon (Department of Plant Pathology, Institute of Agricultural Sciences, Office of Rural Development) ;
  • Chung Bong Jo (Department of Plant Pathology, Institute of Agricultural Sciences, Office of Rural Development)
  • 이순형 (농촌진흥청 농업기술연구소) ;
  • 이기운 (농촌진흥청 농업기술연구소) ;
  • 정봉조 (농촌진흥청 농업기술연구소)
  • Published : 1977.06.01
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Abstract

Potato virus X was purified especially for the preparation of antisera for diagnosis and identification. Potato virus X was isolated Iron infected plants by means of indicator plants and identified in electron microscopy. Isolated PVX was multiplied in tomato plants and purified by a modified procedures. The purity of PVX was 0.59mg/m1. Purified PVX was injected into rabbits once a week for 5 weeks. Antiserum was collected 10 days after the last injection. Produced antiserum was determined 1/1024 titers by means of microprecipitin tests and showed sharp reactions in agar gel-diffusion tests.

감자바이러스X(PVX)의 진단과 동정을 위하여 PVX를 순수분리 순화하여 항혈청을 제조하였다. PVX는 지표식물과 전자현미경으로 순수분리하여 도마도애서 증식한 개량된 방법으로 순화하었다. 순화된 PVX의 순화도는 0.59mg/ml이었으며, 이를 1-2ml씩 7일 간격으로 5회 토끼에 주사하였다. 마지막 주사후 10일에 채혈하여 항혈청을 분리하였다. 제조된 항혈청의 력가는 미량침강법에서 1/1024이었고. 한천내확산법에서도 판명한 반응을 관찰할 수 있었다.

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