농산폐자원의 미생물학적 이용에 관한 연구 (제8보) 섬유질단세포단상질 생산의 시험공장조업

Studies on Microbial Utilization of Agricultural Wastes (Part 8) Pilot Plant Operation for the Production of Cellulosic Single Cell Protein

섬유소 단세프 단백질의 대량생산 시험을 위하여 pilot plant 규모의 시험공장 연구를 수행하였는바 그 결과를 요약하면 다음과 같다. 1) 시험공장에서 균체생산을 위한 최적 조건을 pH 7.4, 온도 34$^{\circ}C$, 교반량 130rpm, 통기량 0.4~0.6VVM이었다. 2) 최적배양 조건에서 배지의 적정 수준은 기질로서 볏짚이 4%, (NH$_4$)$_2$SO$_4$0.2%, $K_2$HPO$_4$0.05%, KH$_2$PO$_4$0.10%, NaCl 0.2%, MgSO$_4$ㆍ7$H_2O$ 0.01%, CaCl$_2$ㆍ2$H_2O$ 0.01%, C. S. L. 0.02%였다.

The cultivation of C. flavigena KIST 321, capable of utilizing cellulosic resources, was carried out in a 500 L fermentor by the batch process and the productivities of cellulosic SCP have been investigated by establishing the optimal conditions and levels of cellulosic material and others as medium components. The highest yield of the cell mass in the batch process was atttained under tile conditions at 30$^{\circ}C$, pH 7.4, 0.4∼0.6 VVM of aeration and at 130 rpm of agitation. According to the material balance of cellulosic SCP production using tile pretreated rice straw as a carbon source, more than 25 percent of rice straw on the base of drying weight was recovered in the form of cell mass.