Chemical Properties of Porcine Leukocyte Lysosomal Hydrolases

Porcine Leukocyte Lysosomal Hydrolases의 화학적성질(化學的性質)에 관(關)한 연구(硏究)

Lysosomal enzyme latency was demonstrated for hydrolases from porcine leukocyte by suspending sediment sfrom differential centrifugation in 0.125 to 0.250 M sucrose. Specific activities pH optima and activation energies were determined for hydrolases distributed in various sedimentation fractions and for enzymes solubilized by n-butyl alcohol extraction. Specific activities of the hydrolases revealed the heterogeneity of the Iysosomal fractions relative to enzyme content. pH optima identified the enzyme as acid hydrolases with optima for cathepsin D and aryl sulfatase also at pH 6.8. Activation energies of some hydrolases were low revealing that these enzymes could function efficiently during low temperature aging of meat.

돼지 백혈구(白血球) Iysoromal enzyme의 latency를 서로 다른 농도의 sucrose용액(0.0125-0.25M)으로서 조사하고 각(各) sedimentation fraction에 분포되어 있는 효소들의 specific activity, pH optima 및 activation energy를 측정하였다.