The Studies on Hydrolysis of the Silk Fibroin by Proteolytie Enzyme, Bombyx mori II. Relation between the Fibroin Hydrolysis of Different Cocoon Layers and Physical Property of Silk Fiber

견 Fibroin의 효소분해에 관한 연구 II-견층부위별 Fibroin 분해율 차이와 견사물성과의 관계-

The study was carried out to investigate the variations in the fibroin hydrolysis of different cocoon shell layers, and the relationship between the hydrolysis and silk physical property. The obtained results are as follows: 1. The fibroin hydrolyzing ratio was highest at the inner layer of cocoon shell and. next in order. at the middle layer and at the outer layer. 2. The fibroin hydrolyzing ratio of abnormal cocoons was higher than normal cocoon and it was different among them, tile highest was double coroon, thin shell cocoon and perforated cocoon in order. 3. The sericin content and fibroin hydrolyzing ratio of Jam 111 and Jam 112 was higher than that of Jam 111 $\times$ Jam 112. 4. The fibroin hydroiyzing ratio of the cocoons fed with the artificial diets was increased at the inner layer. The sericin content of those lessened at the inner layer, however, it slightly increased at the most inner layer more than at the inner layer. 5. The breaking strength of the degummed silk fiber of different cocoon layers was reduced at the inner layer. The breaking Strength of abnormal silk fiber was less than that of normal silk fiber. 6. A negative correlation (r= －0.8) )was approved between fibroin hydrolyzing ratio and breaking strength of silk fiber, and the regression line was Y=－0.29x＋5.07.

각종 고치의 견층부위별 fibroin 효소분해율의 차이 및 견사물성과의 관계를 구명코자 시험하여 다음과 같은 결과를 얻었다. 1. 각종 고치의 견층부위별 fibroin 효소분해율은 외층에서 내층으로 갈수록 높았다. 2. 이상용견의 견층부위별 fibroin 분해율은 정상견에 비하여 높았으며 옥견＞박피견＞사용견＞정견 순위를 보이었다. 3. 원종인 잠111과 잠112의 fibroin 분해율 및 sericin 함유량은 교유종인 잠 111$\times$잠112보다 높았다. 4. 인공사료육잠견의 견층부위별 fibroin의 분해율은 외층보다 내층으로 갈수록 증가되었으며 sericin 함양은 외층에서 내층으로 갈수록 감소되었으나 최내층에서 약간 증가되는 경향을 보이었다. 5. 견층부위별 연편사의 절단강력은 외층에서 내층으로 갈수록 감소되는 경향이었고 이상용견의 절단강역은 정견에 비하여 저하되었다. 6. 견층부위별 fibroin 분해율과 견사의 절단강력과는 부의 상관(${\gamma}$=－0.8)이 인정되고 그 회귀방정식은 y= －0.29x＋5.07 이었다.