Journal of Nutrition and Health
- Volume 9 Issue 3
- /
- Pages.8-17
- /
- 1976
- /
- 2288-3886(pISSN)
- /
- 2288-3959(eISSN)
A Study on Estimation of Minimum Protein Requirement
단백질(蛋白質) 최저요구량(最低要求量) 측정(測定)에 관한 연구(測定)
- Wang, Soo-Kyung (Department of Food and Nutrition, College of Home Economics, Ewha Womans University) ;
- Kim, Mi-Kyung (Department of Food and Nutrition, College of Home Economics, Ewha Womans University)
- Published : 1976.09.30
Abstract
This study was carried out to estimate the minimum protein requirement for the Korean college girls, and to see the effect of carbohydrate intake on the minimum protein requirement in the rat. In the first experiment, three students attending Ewha Womans University were given protein free diet for 7 days while caloric intake were adjusted to maintain constant body weight. Endogenous nitrogen excretion was measured to estimate their minimum protein requirement. Second experiment was carried out in 3 parts to find out the effect of carbohydrate intake on the minimum protein requirement using 15 female, 50 days old rats weighing 120g. Inexperiment 2-1, the rats were fed protein free diet for 9 days and endogenous nitrogen excretion was measured to determine the minimum protein requirement of the rats. In experiment 2-2, the minimum casein requirement of the rats needed to maintain nitrogen equilibrium was estimated by the least squares regression method feeding 3 different levels of casein determined from the result of experiment 2-1. Finally, in experiment 2-3, the rats were given the minimum casein requirement for 7 days with 3 different levels of carbohydrate to find out the effect of carbohydrate intake on the nitrogen balance. The results of this study were as follows. Experiment I. Daily endogenous nitrogen losses of 3 college girls were 1.89g in urine, 1.10g in feces and 0.0108g from skin since the total daily endogenous nitrogen loss was 3.0g and the total daily nitrogen intake was 0.52g, the minimum nitrogen requirement of these subjects was about 2.48g/day (15.5g protein/day). Experiment II. Experiment 2-1: daily endogenous nitrogen losses of the rats fed protein free diet were
Keywords