Studies on tissue culture of medicinal plants (II) -Tissue cultures of Glycyrrhiza glabra L. var. glandulifera $R_{EG.}$ et $H_{ERDER}$-

약용식물(藥用植物)의 조직배양(組織培養)에 관(關)한 연구(硏究)(II) -이태리감초(甘草)의 조직배양(組織培養)-

The callus formation of Glycyrrhiza glabra L. var. $glandulifera\;R_{EG}.\;et\;H_{ERD}$. in tissue culture was promoted on Murashige and Skoog's basal solution supplemented with 40g/l of sucrose, 1mg/l of kinetin and 5mg/l of 2, 4-D. The fresh and dry weights of callus and glycyrrhizin contents in callus of the Glycyrrhiza glabra L. var. $glandulifera\;R_{EG}.\;et\;H_{ERD}$. were determined monthly up to 12 months and obtained the results as follows: 1.The fresh weight of formed callus was increased rapidly from 2 to 4 months but growing rate of callus was slow from 4 to 6 months. This indicates that the cell division of callus was most active during the first $2{\sim}3$ months. 2. Glycyrrhizin contents in callus were also increased but the contents were not related to the increased weight of callus.