Studies on Ultrastructure and Virus Infection of Aspergillus ochraseus

Aspergillus ochraseus의 미세구조(微細構造) 및 바이러스 감염(感染)에 관(關)한 연구(硏究)

These studies were carried out to detect the presence of infected virus- like particles and also were observed the ultrastructures of Aspergillus ochraseus isolated from kokja and Korean ginseng. The results of ultrastructures of Aspergillus ochraseus are summarized as follows: 1. In fungal cells, nuclei were enclosed by a irregular double membrane and nucleoli in the nucleus. 2. In cytoplasm, mitochondria, rough endoplasmic reticulum with ribosomes and glycogen were scattering distributed and many lomasomes also observed. 3. The osmiophilic bodies of fungal cells existed in the vesicles. 4. The cell walls were composed of a low electron dense materials. 5, Conidia cell walls were extremely thick and possessed the high electron density of outer coat. The virus-like particles were observed in the hyphae of Penicillium chrysogenum Q-176. These virus-like particles measured $350{\AA}$ in diameter. But strains of Aspergillus ochraseus, showing some vesicle particles were also observed about $800{\AA}$ in diameter in the central region of young fungal hyphae. Based on the results of these experiments, it can not be determined virus particles or not. The further studies to determination of virus particles will be proceeded by the chemical, physical and biological assay methods.