Korean journal of applied entomology (한국응용곤충학회지)

Korean Society of Applied Entomology (한국응용곤충학회)
권호 표지

Serological Investigation of Virus Diseases of Tobacco Plant (Nicotiana tabaccum L.) In Korea

혈청학적 방법에 의한 잎담배 바이러스병의 감염상 조사

  • Park Eun Kyung (Bucheon Tobacco Experiment Station, Office of Monopoly) ;
  • La Yong Joon (College of Agriculture, Seoul National University) ;
  • Heu Il (Bucheon Tobacco Experiment Station, Office of Monopoly) ;
  • Lee Yong Deuk (Bucheon Tobacco Experiment Station, Office of Monopoly)
  • 박은경 (전매청 부천연초시험장) ;
  • 나용준 (서울대학교 농과대학) ;
  • 허일 (전매청 부천연초시험장) ;
  • 이용득 (전매청 부천연초시험장)
  • Published : 1975.05.01
Download PDF
⟨ Previous Next ⟩

Abstract

A total of 40 virus infected tobacco plants (Nicotiana tabaccum L.) with various symptom types Were collected from Bucheon and Jeonju area by its symptoms were investigated on the incidence of tobacco mosaic virus (TMV), cucumber mosaic virus (CMV), alfalfa mosaic virus (AMV), potato virus X (PVX) and potato virus Y (PVY) by serological methods. van Slogteren's microprecipitin test was applied for the testing of PVX and PVY from infected plants and Ouchterlony agar double diffusion test was used for CMV, TMV and AMV. Results obtained are as follows: 1. TMV, CMV, AMV, PVX and PVY wcre found to occur on the tobacco plants growing in Korea. 2. The prevalence of each of these viruses among the 40 tobacco plants investigated was in the order of AMV: $(67.5\%)>CMV:(60.0\%)>TMY:(47.5\%)>PVY:(17.5\%)>PVX: (10.0\%).$ 3. In Burley variety, the percentage of infection by TMV was $15\%$, whereas it was as high as $80\%$ in Hicks variety. 4. Among the 40 tobacco plants investigated, $37.5\%$ showed infection with one kind of virus whereas the remaining $62.5\%$, revealed mixed infection with more than two different viruses.

우리나라 잎담배에 발생하고 있는 바이러스의 종류와 감염상을 조사하기 위하여 전주지방에서 Burley종 (Burley-21) 20개체, 부천연초시험장 포장에시 황색종(Hicks) 20개체를 병징별로 채집하여 혈청학적인 방법에 의해 실시했다. 검정대상 바이러스는 Tobacco mosaic virus(TMV), Cucumber mosaic virus(CMV), Alfalfa mosaic virus (AMV), Potato virus X(PVX) 및 Potato virus Y(PVY)등 5종으로 TMV, AMV, CMV는 한천내확산법으로 PVX, PVY는 미량침강법으로 실시하여 다음과 같은 결과를 얻었다. 1. 공시이병개체중에서 TMV, CMV, AMV, P X 및 PVY 등 5종의 바이러스가 검출되었다. 2. 조사된 40개체중 각 바이러스의 감염율은 AMV $67.5\%$, CMV $60.0\%$, TMV$47.5\%$PVY$17.5\%$, PVX$10.0\%$의 순이었다. 3. Burley종에서는 TMV감염율이 $15.0\%$로 황색종의 $80.0\%$보다 현저히 낮았다. 4. 조사된 각 개체는 단독감염으로부터 2-4종의 바이러스로 다양하게 혼합감염을 나타냈으며 이 중 단독감염이 $37.5\%$, 혼합감염이 $62.5\%$이었다.

Keywords