Serological Study on Rice Stripe Virus

벼 줄무늬잎마름병 바이러스에 대한 혈청학적인 검토

This experiment was performed to clarify the concentration of rice stripe virus in the rice Plant leaves by serological test, and was attempted to inspect the virus carrier among small brown planthopper by antibody-sensitized hemagglutination test. The antiserum was prepared by injecting intervenously into the external marginal vein of the ear of a rabbit. The precipitin titer of it was 1 : 16. The rough virus fluid prepared from diseased leaves was centrifuged at 10.000 rpm, and then the supernatant solution was treated at $55^{\circ}C$ for 5 minutes and the solution clarified by removing the agglutinate was used as the antigen solution. Antibody-sensitized erythrocyte solution was prepared from sheep erythrocytes sensitized by rice stripe virus with tannic acid, and its agglutination titer was 1 : 512. The virus concentrations in flag leaves or first leaves just below them showing different symptoms was high with progressing the severity of symptoms. And the concentrations of the virus in leaves of varieties of the rice plant showing same degree symptom were lower in suscetible varieties, Sadominori, Palgoeng, Mangyong and Nihonbare, than in the resistant one, Tongil, but in Yooshin which was known as the resistant, lower rather than in Tongil. The reacton of antibody-sensitized hemagglutination test to inspect the virus carrier, was so highly sensitive that this reaction was recognized as a method which is able to Identify the carrier accurately in short time.

벼줄무늬잎마름병 바이러스에 대한 혈청학적인 검토결과를 다음과 같이 적요한다. 1. 벼줄무늬잎마름병 바이러스는 항원성을 갖고 있으며 항체와 특이적인 혈청반응을 타나냈다. 2. 가토 정맥주사에 의한 항혈청의 항체가는 아주 낮아 16배에 불과하였다. 3. 벼 품종 $\ulcorner$사도미노리$\lrcorner$에 있어서 침강반응혼합법으로 측정한 결과 이병엽의 병징정도에 따라 바이러스 농도에 차이가 인정되는데 병징정도가 심할수록 바이러스농도가 높았다. 4. 동정도의 병징이라도 벼품종에 따라 바이러스농도가 달랐는데 저항성품종인 $\ulcorner$통일$\lrcorner$에서는 높았고 이병성인 $\ulcorner$사도미노리$\lrcorner$, $\ulcorner$팔굉$\lrcorner$, $\ulcorner$만경$\lrcorner$, $\ulcorner$니혼바레$\lrcorner$에서는 낮았다. 그러나 저항성 품종인 $\ulcorner$유신$\lrcorner$에서는 낮았다. 5. 항체감작적혈구 응집반응에 의한 항체가는 아주 높아 512배였으며 보독충의 검정시험에서도 반응이 잘 나타나 $38\%$라는 보독충율을 나타냈다.