Studies on the Grape Wine (Part 1) - The isolation and identification of grape wine yeasts

포도주에 관한 연구 (제1보) - 포도주효모(酵母)의 분리(分離) 및 동정(同定)

  • Park, Y.J. (Dept. of Food Technology, processing College of Agriculture, Chungnam National University) ;
  • Yoon, H.K. (Dept. of Food Technology, processing College of Agriculture, Chungnam National University) ;
  • Lee, S.K. (Dept. of Food Technology, processing College of Agriculture, Chungnam National University) ;
  • Yoon, B.H. (Dept. of Food Technology, processing College of Agriculture, Chungnam National University)
  • 박윤중 (충남대학교 식품가공학과) ;
  • 윤한교 (충남대학교 식품가공학과) ;
  • 이석건 (충남대학교 식품가공학과) ;
  • 윤복현 (충남대학교 식품가공학과)
  • Published : 1975.12.31

Abstract

These experiments were carried out to obtain useful yeast for Korean grape wine brewing. The 213 strains of yeasts were isolated from grapes sampled in Daejeon city and suburbs and then 3 strains among the isolated yeasts were selected as useful strains and identified. The results obtained as follows; 1. The 3 strains of W-49, W-50 and W-127 selected as useful yeasts were clarified to grape wine mash rapidly. 2. In the mashes clarified after storage of 45 days, the value of optical density of the case of W-50 strain was highest and the color was very thick, compared with the case of another strains. 3. In the pannel test of the grape wines, the case of W-49 and W-50 strains were best, the case of the W-127 strain and control yeast, Hb were similar each other as next order. 4. Among 3 strains selected as useful yeasts it were identified that the strains of W-49, W-50 selected as useful strains were Saccharomyces cerevisiae and the strain of W-127 was Saccharinyces pretoriencis by the Ladder manual. 5. All of these useful yeasts were appeared red color on TT C stain.

한국산(韓國産) 포도주 양조(釀造)에 적합(適合)한 우수균주(優秀菌株)를 얻기 위(爲)하여 대전시(大田市) 일원(一圓)에서 수집(蒐集)한 시료(試料) 포도 중(中)에서 213주(株)의 효모(酵母)를 분리(分離)하여 이들 중에서 우수(優秀)한 균주(菌株)로서 3주(株)를 선정(選定)하여 실험(實驗)한 결과(結果)는 다음과 같다. 1. 우수균주(優秀菌株)로 선정(選定)된 W-49, W-50 및 W-127은 포도주 양조(釀造)에 있어서 발효료을 속(速)히 청징화(淸澄化)하였다. 2. 저장(貯藏) 45일(日) 후(後) 청징화(淸澄化)된 포도주들의 $OD^{530}_{10}$를 비교(比較)할때 W-50 균주(菌株)의 것은 OD치(値)가 크고 색도(色度)가 매우 짙었다. 3. 관용시험(官龍試驗) 결과(結果) W-49와 W-50의 것이 가장 좋았으며 W-127의 것은 그 다음 순(順)으로서 대조(對照) 균주(菌株) Hb의 것과 비슷하였다. 4. 우수균주(優秀菌株)의 균학적(菌學的) 성질(性質)을 살펴 Lodder의 분류동정법(分類同定法)에 따라 동정(同定)한 결과(結果) W-49와 W-50의 균주(菌株)는 Saccharomyces cerevisiae로 동정(同定)되었으며 W-127의 균주(菌株)는 Saccharomyces pretoriensis로 동정(同定) 되었다. 5. 이들 우수균주(優秀菌株)의 TTC 정색(呈色) 반응(反應)은 모두 TTC Red 효모(酵母)로 나타났다.

Keywords

Acknowledgement

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