생물체(生物體)로부터 천연화합물(天然化合物) 추출(抽出)에 관한 연구(硏究) 제(第)II보(報) : 지방(脂肪)을 제거한 치자로부터 치자색(色) 색소(色素) 추출(抽出)에 관한 연구(硏究)

Studies on the Extraction of Natural Compounds from Plants and Microorganisms Part 2. Extraction of Orange-yellow Pigment from Defatted Gardenia

미쇄(微碎)한 치자분말(粉末)에서 Orange-Yellow 색소(色素)를 추출(抽出)하면 짧은 시간(時間)에서 $log\;t^{1{\cdot}15}$에 비례(比例)하였고 최적(最適) 추출시간(抽出時間)은 30분(分)이 선정(選定)되었으며 온도(溫度)가 상승(上昇)함에 따라 $log\;T^{3{\cdot}73}$에 비례(比例)하였고 최적(最適) 추출온도(抽出溫度)는 $40^{\circ}C$가 선정(選定) 되었으며 시료(試料) 0.1 g당(當) 용매량(溶媒量)의 증가(增加)에는 $log\;S^{3{\cdot}7}$의 색소(色素) 추출량(抽出量)을 나타냈고, 지방(脂肪)을 완전(完全)히 제거(除去)한 시료(試料)는 전혀 제거(除去)하지 않은 시료(試料)보다 $0.6(O.D\;448\;m{\mu})$만큼의 좋은 추출효과(抽出效果)를 보였다. 이들을 綜合하여, $P_{total}{\simeq}1.15\;log\;t+3.73\;log\;T+3.7\;log\;S-4X-6.4$ $P_{total}$: total amounts of extracted orange-yellow pigment from Gardenia. t: extraction time $(0.5{\sim}60\;min)$ T: extraction temperature $(5{\sim}50^{\circ}C)$ S: volume of solvent $(5{\sim}50\;ml)$ X: fat contents $(0{\sim}15%)$ 와 같은 수정(修正)된 실험식(實驗式)을 유도했다.

The extraction efficiency of orange-yellow pigment from the Gardenia was greatly depended upon the extraction time, extraction temperature, volume of solvent used and fat contents of the Gardenia. From the experimental results, the amounts of extracted pigment (P) was proportional to the $log\;t^{\;1{\cdot}15}$ of extraction time$(t;\;0{\sim}60\;min.)$, the $log\;T^{3{\cdot}73}$ of extraction temperature$(T;\;5{\sim}60^{\circ}C)$, the $log\;S^{3{\cdot}7}$ of volume of solvent$(S;\;5{\sim}50\;ml)$, and the -4X of fat contexts of sample $(X;\;0{\sim}0.\;15)$ at $18^{\circ}C$ for 10 minutes. Finally, the modified empirical equation was derived as follow; $P{\simeq}1.15\;log\;t+3.73\;log\;T+3.7\;log\;S-4X-6.4$ In addition to that, the most optimum conditions of pigment extraction were determined as 30 minutes of operation time, $40^{\circ}C$ of temperature. Deffated Gardenia was more productive than natural Gardenia in the pigment extraction.