Korean Journal of Veterinary Research (대한수의학회지)
- Volume 14 Issue 2
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- Pages.243-252
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- 1974
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- 2466-1384(pISSN)
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- 2466-1392(eISSN)
Experimental Studies on Detection of Salmonellae in Animal-origin Foods by Means of Dirct Fluorescent Antibody Technique
직접형광항체법(直接螢光抗體法)에 의한 축산식품중(畜産食品中)의 Salmonella 균(菌) 검출(檢出)에 관한 실험적(實驗的) 연구(硏究)
- Jeon, Moo Hyeong (Institute of Veterinary Research, Office of Rural Development) ;
- Cha, Youn Ho (Institute of Veterinary Research, Office of Rural Development) ;
- Chung, Gill Taik (Department of Veterinary Medicine, College of Agriculture, Seoul National University)
- Published : 1974.10.15
Abstract
The experiment was performed in order to investigate the applicability of the rapid detection of salmonellae in various animal-origin foods by means of the direct fluorescent antibody technique. Egg, sausage and chicken were inoculated with various concentrations of Sal.paratuphi A, Sal. paratyhi B and Sal. thompson, and the fluorescent antibody technique was applied and compared with the conventional cultura method for the sensitivity of detection of the organisms. Two methods were employed in the fluorescent antibody technique; the direct smear method in which the smear being made directly from the specimens, and the enrichment smear method in which the smear being made from the enrichment broth. The effect of various enrichment time (1,5,8,11 and 13 hours) in tetrathionate broth on the detection of salmonellae in the fluoresent antibody technique was also studied. The results obtained were summarized as followings; 1. Of the three methods, the enrichment smear method of fluorescedt antibody technique was highly effective as cultural method for the detection of salmonella organisms. 2. Direct smear method of fluorescent antibody technique was effective as two other methods
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