Korean Journal of Soil Science and Fertilizer (한국토양비료학회지)

Korean Society of Soil Science and Fertilizer (한국토양비료학회)
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Effects of Nitrogen Sources on Chlorophyll, and Oxidases in Soybean Leaves different in Phosphorus Sensitivity

인산감수성(燐酸感受性)이 다른 대두엽(大豆葉)의 엽록소(葉綠素) 및 산화효소(酸化酵素)에 대(對)한 질소원(窒素源)의 영향(影響)

  • Park, Hoon (Institute of Agricultural Sciences, Office of Rural Development) ;
  • Stutte, Charles A. (Department of Agronomy, University of Arkansas Faytteville)
  • Received : 1973.06.06
  • Published : 1973.09.20
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Abstract

Effects of nitrogen sources on chlorophyll, activity of some oxidases in soybean leaves were investigated in relation to phosphorus sensitivity. Ammonium and urea culture accelerated leaf senescence more in phosphorus sensitive cultivars. The leaf senescence patterns affected by nitrogen sources were clearly indicated by intact leaf absorbance. Absorption maximum (670nm in methanol extraction or 685nm in intact leaf), was not changed by nitrogen source in the same method. According to leaf senesence pattern general physiological sensitivity pattern was discussed. IAA-oxidase activity was higher in the phosphorus tolerant cultivars and nitrate treatment than in the sensitive cultivars and ammonium treatment. Glycolate oxidase activity was higher in the sensitive cultivars and nitrate treatment. Polyphenol oxidase activity was higher in the tolerant cultivars and urea treatment. It is concluded that the excess ammonium, to which excess phosphorus is highly similar in physiological effect, disturbs the photosynthetic system by inhibition of ATP generation (photo-and oxidative phosphorylation).

대두(大豆)의 인산감수성(燐酸感受性)과 관련(關聯)하여 엽중(葉中)의 엽록소(葉綠素) 및 산화효소활성(酸化酵素活性)에 대(對)한 질소원(窒素源)의 영향(影響)을 조사(調査)하였다. 암모니움태(態)와 요소태(尿素態) 배양(培養)은 인산감수성(燐酸感受性)이 큰 품종(品種)에서 엽(葉)의 노화(老化)를 촉진(促進)시켰다. 질소원(窒素源)에 의(依)한 엽(葉)의 노화양상(老化樣相)은 생엽자체(生葉自體)의 흡광도(吸光度)로 명확(明確)하게 나타났다. 최고흡광파장(最高吸光波長)(메타놀 추출(抽出)에서 670nm, 생엽자체(生葉自體)에서 685nm)은 동일(同一)한 방법(方法)에서는 질소원(窒素源)에 의(依)하여 변(變)하지 아니하였다. 엽(葉)의 노화양상(老化樣相)에 의(依)하여 생리적(生理的) 감수성(感受性)의 일반적(一般的) 양상(樣相)을 검토(檢討)하였다. IAA-oxidase 활성(活性)은 초산태(硝酸態) 배양(培養)과 내인성품종(耐燐性品種)에서 암모니움 태배양(態培養)과 감수성(感受性) 품종(品種)에서 보다 컸다. Glycolate oxidase 활성(活性)은 감수성(感受性) 품종(品種)과 초산태배양(硝酸態培養)에서 높았다. Polyphenol oxidase 활성(活性)은 내인성(耐燐性) 품종(品種)과 요소배양(尿素培養)에서 컸다. 암모니움 과잉(過剩)은 ATP 생성(生成)(광인산화(光燐酸化) 및 산화적인산화(酸化的燐酸化))을 억제하여 광합성(光合性)을 조해(阻害)하고 인산과잉(燐酸過剩)도 암모니움 과잉(過剩)에 매우 유사(類似)한 것으로 결론(結論)되었다.

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