The Effects of Penicillin on the growth of Bacillus subtilis

Penicillin이 Bacillus subtilis의 생육에 미치는 영향

This experiment was done to investigate the growth and the fine structural changes of Bacillus subtilis which were influenced by the penicillin with electromicroscope. The results were as follows; 1) The higher the concentration of penicillin the more prominent inhibition of the growth was observed. 2) The septa was not formed, derangements of synthesis of cell wall and cell membrane. 3) Cytoplasm was increased with swelling of cell body because of weakness of cell membrane induced by deranged synthesis of cell membrane. Some of the cells showed disruption of their membrane with loss of cytoplasm, remaining empty space, which suggest loss of cell function. 4) It can be suggested that penicillin had affected on the cell wall of Bacillus subtilis, and inhibited growth of the cell by deranging the formation of the cell wall.

Penicillin을 농도별로 첨가한 Bouillon medium에서 배양한 Bacillus subtilis의 성장상태와 세포내미세구조를 전자현미경으로 관찰하여 다음과 같은 결과를 얻었다. 1. penicillin의 농도가 높아짐에 따라 본균의 성장이 현저하게 억제되고 분열된 세포일지라도 분리가 잘 되지않고 서로 융합되어 있다. 2. Cell wall 및 cell membrane 성장에 저해를 일으켜 septa형성이 불가능하다. 3) 세포질은 증가하나 세포막의 형성저해로 인하여, 세포가 팽대되어 세포막이 파열되며, 세포질이 세포외로 유출되어 내부가 공백상태로 된다. 따라서 그 기능을 상실하게 되는 것 같다.