Effects of Temperature on the Activity of Pulmonary Surfactant of the Rabbit

온도(溫度)가 가토(家兎) 폐포표면(肺胞表面) 활성물질(活性物質)의 활성도(活性度)에 미치는 영향(影響)

Though it has been reported by Clements et al. and Avery et al. that the activity of the pulmonary surfactant can be altered by the temperature changes, a conclusive evidence of the effects of temperature on the surfactant system of the lung is yet to come. In the present study, an attempt was made to observe possible effects of a few different degrees of temperature on the activity of the pulmonary surfactant of the rabbit in vivo and in vitro. The rabbit was sacrificed by blood shedding and both lungs were completely removed. The lung washings, obtained by gently lavaging the left lung with saline, was placed at 1) 4C for 1, 5, 10, 15, 30 and 40 days, and 2) 20C for 1, 2, 3, 4, 5 and 7 days for in vitro experiment. For in vivo experiment, the rabbit was placed at 4C for 4, 8, 12 and 24 hours, and the lung lavage was prepared as described above in the in vitro experiment. Tension-area (T-A) diagram of the lung lavage was recorded automatically by a modified. Langmuir-Wilhelmy balance with a synchronized recording system. The surface tensions thus obtained were compared with those of the normal rabbit, and the results are summarized as follows: 1. The maximal surface tension, minimal surface tension and stability index of the normal rabbit lung lavage were $52.5{\pm}2.3\;dynes/cm,\;4.9{\pm}2.3\;dynes/cm$ and 1.65, respectively. 2. In the group where the lung lavage was placed at 4C in vitro, the maximal and minimal surface tensions, and stability index did not show any noticeable changes comparing with the normal values up to 30 days. On the 40th day of the experiment, a tendency of a slight increase in the surface tensions was observed but the change was not significant. 3. When the lung lavage was placed at 20 C in vitro, the maximal surface tension did not show any appreciable change comparing with the normal except on the 7 th day with a slight increase. The minimal surface tension showed an increased value from the 2nd day, and on the 5 th and 7 th experimental day, markedly increased value was observed. The stability index, on the other hand. showed a marked decrease throughout the entire experiment with the value of 0.71 and 0.53 on the 5th and 7 th day, respectively. 4. In the group where the rabbit was placed at 4 C in vivo, the maximal surface tensions and stability index of the lung lavage showed little change from the normal. The minimal surface tension at 12 experimental hour showed a slight increase, but it returned to the normal value at 24 hour.

온도(溫度)에 대(對)한 폐포표면활성물질(肺胞表面活性物質)의 활성도(活性度)의 태도(態度)를 구명(究明)코져 가토(家兎)의 좌측(左側) 폐장(肺臟)을 적출(摘出)하여 생리적(生理的) 식염수(食鹽水)로 폐포세척액(肺胞洗滌液)을 작성(作成)하여 4 C에 1, 5, 10, 15, 30 및 40일간(日間) 방치(放置)한 군(群)과 20 C에 1, 2, 3, 4, 5 및 7일간(日間) 방치(放置)한 군(群) 및 정상가토(正常家兎)를 4 C에 4, 8, 12 및 24 시간(時間) 노출(露出)한 후(後) 좌측(左側) 폐장(肺臟)을 적출(摘出)하고 폐포세척액(肺胞洗滌液)을 작성(作成)한 군(群)에서 그 표면장력(表面張力)을 측정(測定)하여 정상가토(正常家兎)의 그것과 비교(比較) 결과(結果)는 다음과 같다. 1) 정상가토(正常家兎)의 폐포세척액(肺胞洗滌液)의 최대(最大) 및 최소표면장력(最小表面張力)및 stability index는 각각(各各) $52.5{\pm}2.3,\;4.9{\pm}2.3$ dynes/cm 및 1.65였다. 2) 4 C에 1, 5, 10, 15, 30 및 40일간(日間) 방치(放置)한 폐포세척액(肺胞洗滌液)의 최대(最大) 및 최소표면장력(最小表面張力) 및 stability index는 각각(各各) 정상군(正常群)의 그것과 비교(比較)하여 큰 변화(變化)가 없었으며 40일군(日群)에서는 약간(若干) 증가(增加)하는 경향(傾向)을 보였으나 의의(意義)있는 변화(變化)는 아니었다. 3) 20 C에 1, 2, 3, 4, 5 및 7일간(日間) 방치(放置)한 폐포세척액(肺胞洗滌液)의 최대표면장력(最大表面張力)은 뚜렷한 변화(變化)를 보이지 않았으나 제 7 일(第 7 日)에 약간(若干) 증가(增加)하였으며 최소표면장력(最小表面張力)은 제 2 일(第 2 日)부터 증가(增加)하는 경향(傾向)을 나타내어서 제(第) 5 및 제 7 일(第 7 日)에는 현저(顯著)히 증가(增加)하였으며 그 stability index는 현저(顯著)히 감소(減少)하여 제(第) 5 및 제 7 일(第 7 日)에는 각각(各各) 0.71 및 0.53이었다. 4) 4 C에 4, 8, 12 및 24시간(時間) 노출(露出)한 가토(家兎)에서 얻은 폐포세척액(肺胞洗滌液)의 최대(最大) 및 최소표면장력(最小表面張力) 및 stability index는 각각(各各) 정상군(正常群)의 그것과 비교(比較)하여 큰 변화(變化)를 나타내지 않았으나 제(第)12시간(時間)에서 최소표면장력(最小表面張力)이 약간(若干) 증가(增加)하는 경향(傾向)을 보였으며 제(第)24시간(時間)에는 다시 정상군(正常群)의 그것과 비슷한 경향(傾向)을 나타내었다.