Journal of the Korean Chemical Society (대한화학회지)

Korean Chemical Society (대한화학회)
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An ESR Study of Amino Acid and Protein Free Radicals in Solution. Part IV. An ESR study of Gamma-Irradiated Amino Acids in Frozen Aqueous Solutions.

  • Sun-Joo Hong (Department of Biochemistry and Biophysics, University of Hawaii) ;
  • D. E. Holmes (Department of Biochemistry and Biophysics, University of Hawaii) ;
  • L. H. Piette. (Department of Biochemistry and Biophysics, University of Hawaii)
  • Published : 1971.10.30
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Abstract

An ESR study has been made on free radicals produced in frozen aqueous solutions (ices) of glycine, DL-${\alpha}$-alanine, DL-serine, L-cysteine, DL-leucine and DL-isoleucine by gamma-irradiation at dry ice temperature. All free radicals induced were decayed concomitant to the successive annealing but the radical species which is believed to be dominant seems to be stable even near the melting point of the ice. These dominant species were found to be identical to those resulted from direct action of radiation in the solid at room temperature. Small but significant changes in the spectra of glycine and DL-${\alpha}$-alanine were observed by varying the microwave power. These results seem to support the view that the spectra obtained were composite consisting of more than two different resonances having different power saturation characteristics. The relative contribution of unidentified resonances to the composite spectra was greater for solutions of low concentration. These resonances are assumed to be induced by indirect effects, mainly hydrogen abstraction by radiation produced hydroxyl radicals and also C-N bond cleavage by hydrated electrons.

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References

  1. Radiation Res. v.17 T. Henriksen
  2. J. Chem. Phys. v.38 T. Henriksen
  3. Radiation Res. v.25 T. Sanner
  4. Abstracts, 16th Radiation Research society Annual Meeting, Paper No. Dc-5 Sun-Joo Hong;D. E. Holmes;L. H. Piette
  5. The fifth international symposium of free radicals H. Engelhard;G. Breuer;Friedrich-Wilhelm Froben
  6. This Journal v.15 Sun-Joo Hong;L. H. Piette
  7. Korean Biochem. J. Sun-Joo Hong;L. H. Piette
  8. J. Chem. Phys. v.34 S. Siegel;J. M. Flournoy;L. H. Baum
  9. Nature v.193 T. Henriksen
  10. J. Chem. Soc. D. K. Ghosh;D. H. Whiffien
  11. Int. J. Rad. Biol. v.7 A. Muller;P. E. Schambra;E. Pietsch
  12. Radiation Res. v.29 W. Snipes;J. Schmidt
  13. J. Chem. Phys. v.42 T. Cole;H. C. Heller
  14. J. Am. Chem. Soc. v.88 H. Box;H. G. Freund;E. E. Budzingki
  15. Radiation Res. no.Suppl., 4 W. M. Garrison
  16. Radiation Res. v.32 R. L. S. Willix;W. H. Garrison
  17. J. Phys. Chem. v.71 J. W. SincIair;M. W. Hanna
  18. Radiation Res. v.14 F. Patten;W. Gordy
  19. Current topics in readiation research v.1 K. G. Zimmer;A. Muller;M. Ebert(ed.);A. Howard(ed.)
  20. J. Chem. Phys. v.36 T. Henriksen
  21. J. Chem. Phys. v.37 T. Henriksen
  22. J. Phys. Chem. v.71 D. H. Volman;J. Wolstenholme;S. G. Hadley
  23. J. Chem. Phys. v.34 Y. Kurita;W. Gordy