Direct Determination of Serum Zinc by Atomic Absorption Spectroscopy

원자흡광분광분석법에 의한 혈청 Zn의 측정

  • Tchai, Bum-Suk (Department of Biochemistry College of Medicine Seoul National University) ;
  • Suk, Young-Gunn (Department of Biochemistry College of Medicine Seoul National University) ;
  • Park, Tong-Soo (Department of Biochemistry College of Medicine Seoul National University)
  • 채범석 (서울대학교 의과대학 생화학교실) ;
  • 석영건 (서울대학교 의과대학 생화학교실) ;
  • 박동수 (서울대학교 의과대학 생화학교실)
  • Published : 1971.09.30

Abstract

Zinc is an essential trace element. Previos methods for the determination of zinc in serum have been difficult and reported findings must be treated with caution. The normal range of zinc in serum varies significantly according to different methods and author. The scope of this work was to establish the normal range of zinc in serum values in healthy Korean persons. Methods are described for the determination of zinc in serum diluted ten-fold with deionised water using Hitachi Model 207, atomic absorption spectrophotometer. The values of zinc in serum of 23 healthy Korean persons by atomic absorption spectroscopy ranged from 87.6 to $131.2\;{\mu}g/100\;ml$ with the mean value $109.1\;{\mu}g/100\;ml$ and standard deviation 10.86. In this method, satifactory result was obtained with regard to the coefficient variation and recoveries of added zinc in the measurenents.

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