Studies on Cellulolytic Enzymes Produced by Aspergillus saitoi -II. Influence of Various Media Condition on Cellulase Production-

Aspergillus saitoi가 생산(生産)하는 섬유소(纖維素) 분해(分解) 효소(酵素)에 관(關)한 연구(硏究) -제 2 보(第 2 報) 각종(各種) 배지조건(培地條件)이 Cellulase 생성(生成)에 미치는 영향에 관하여-

This experiment was conducted to study on the productive conditions of cellulase by Aspergillus saitoi in the shaking culture medium. The results were as follows: 1. The production of enzyme required higher concentration of corn steep liquor than that of dextrin. 2. The concentration of 1.0% $NH_4H_2SO_4$ produced the enzyme excellently than 3.0%. 3. The cellulase was produced very slowly by adding $(NH_4)_2SO_4$, but the final concentration of the enzyme was higher than control. The production was suppressed by addition of $CaCO_3$. 4. The addition of $1.0{\sim}2.0%$ substrate caused an increase or stimulation in cellulase production.

Aspergillus saitoi 가 생성(生成)하는 cellulase의 생성조건(生成條件)을 진탕배양법으로 실험(實驗)하여 다음과 같은 결과(結果)를 얻었다. 1) Corn steep liquor는 dextrin에 비해서 대량(大量)을 필요(必要)로 하였다. 2) $NH_4H_2PO_4$ 1.0% 첨가가 3.0% 첨가보다 cellulase 생성(生成)이 우수하였다. 3) $(NH_4)_2SO_4$는 효소생성(酵素生成)은 늦고 생성량(生成量)은 대조구보다 높았으며 $CaCO_3$의 첨가는 억제되었다. 4) 기질(基質)의 $1.0{\sim}2.0%$ 첨가(添加)는 효소(酵素)의 생성(生成)을 증가(增加) 또는 촉진(促進) 시킨다.