Applied Biological Chemistry
- 제13권3호
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- Pages.231-235
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- 1970
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- 2468-0834(pISSN)
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- 2468-0842(eISSN)
고온성 사상균의 효소에 관한 연구-(제3보) 고온성 사상균의 Amylase-
Studies on Enzyme of the Thermophilic Mold-Part. 3. Thermophilic mold amylase-
- Chung, Dong-Hyo (Dept. of Fermentation Technology, College of Engineering Kon-Kuk University) ;
- Lee, Ke-Ho (College of Agriculture, Seoul National University)
- 발행 : 1970.12.30
초록
1. 내열성 사상균 Humicola속의 Amylase를 정제하였다. 2. DEAE-Cellulose Column Chromatography로 2개의 활성구분으로 나누어 졌다. 3. 즉 pH 6.0, 0.05M와 0.5M의 인산염완충용액으로 용출시킬때 전자에서 당화형, 후자에서 호정화형 Amylase가 각각 분리되었다. 4. 이 당화형 Amylase의 최적 pH는
1. Purification of amylase system produced from Humicola sp. by a submerged culture eras carried out. 2. By DEAE-Cellulose column chromatography amylase system was separated into two fractions eluted at 0.05M and 0.5M phosphate buffer solution of pH 6.0. 3. The saccharogenic amylase was mostly composed of. the fraction of 0.05M phosphate buffer solution of pH 6.0 while the dextrinogenic amylase was perseted in fraction of 0.5M phosphate buffer solution of pH 6.0 4. It was found that the optimum pH of this saccharogenic amylase was within the range of from 4.5 to 5.5, stable pH was within the range of from 4.0 to 9.0 and optimum temperature was
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