Changes in Hemolymph Protein Concentration and Oxygen Consumption during the Metamorphosis in pieris rapae L.

배추흰나비의 變態에 따른 血蛋白質과 酸素消費量의 變化

Changes in the protein concentration in the hemolymph and the oxidation of respiratory substrates at various developmental stages in cabbage worm, Pieris rapae Linne were measured using Biuret method and Warburg manometric method, respectively. The results are summarized as follows: 1. The hemolymph protein contentration decreased gradually until the six-day pupa and increased thereafter. In the fifth larval instar the concentration reached a maximum and was about two times the value for the six-day pupa. 2. Endogenous respiration was very high at the prepupal stage, decreasing at two-day pupa, followed by an increase at the late pupal stage. 3. Glucose showed the marked activity throughout the whole stages, and had the striking influence on both the prepupal and adult stages. 4. The changes in protein concentration and the rate of oxygen consumption exhibited a general pattern of U-shaped curve through the process of life cycle according to histogenesis of the adult organ and histolysis of the larval organ.

배추흰나비(Pieris rapae Linne)의 變態에 따른 hemolymph protein 의 濃度變化와 酸素消費量 및 5齡의 呼吸基質에 對한 活性度를 各各 Biuret method 와 Warburg manometric method 로 測定하여 形態的 變化를 比較하였다. 1. Hemolymph protein 의 濃度는 終齡幼蟲에서 가장 높으며 變態에 따라 감소하여 용기말에서 다시 增加한다. 2. Endogenous respiration은 전용기에서 가장 높은 酸素消費量을 보이며 變態에 따라 감소하여 용기말에서 다시 增加한다. 3. Glucose 는 全變態期를 通하여 다른 呼吸期質보다도 가장 큰 活性을 보이며 전용과 용기말에서 현저하게 영향을 미친다. 4. Hemolymph protein 의 濃度變化와 酸素消費量은 全變態期를 通하여 밀접한 연관을 가지며 幼蟲器官의 解消와 成蟲器官의 新生에 따라 U字型을 나타낸다.