Studies on the Glucose Isomerizing Enzyme -Part I. The Isolation and Detection of Glucose Isomerizing Enzyme produced by Microorganism-

포도당(葡萄糖) 이성화(異性化) 효소(酵素)에 관(關)한 연구(硏究) -제1보(第一報), 포도당(葡萄糖) 이성화(異性化) 효소생성균(酵素生成菌)의 분리(分離) 및 검색(檢索)-

  • Seu, J.H. (Dept. of Applied Microbilogy, Agr. College, Kyung-Pook National University) ;
  • Kim, C.K. (Dept. of Applied Microbilogy, Agr. College, Kyung-Pook National University) ;
  • Ki, W.K. (Dept. of Applied Microbilogy, Agr. College, Kyung-Pook National University) ;
  • Rhee, I.K. (Dept. of Applied Microbilogy, Agr. College, Kyung-Pook National University) ;
  • Kwon, T.J. (Dept. of Applied Microbilogy, Agr. College, Kyung-Pook National University) ;
  • Woo, D.L. (Dept. of Applied Microbilogy, Agr. College, Kyung-Pook National University)
  • Published : 1969.04.30

Abstract

With an attempt to obtain a glucose isomerizing enzyme producing microorganism, one hundred and thirty-three strains of microorganism were isolated from soil samples. After screening, a strain K-17 which belonging to actinomyces family, was finally selected. Using this strain of K-17, sugars produced from glucose by the reaction of sugar isomerizing enzyme were tested with paper chromatography. Only a kind of resulting sugar, fructose, was detected from enzyme reaction sample and other sugars were never detected. By these results, the enzyme produced by strain K-17 is classified as a glucose isomerase.

본(本) 연구(硏究)에서 얻어진 결과(結果)를 요약(要約)하면 다음과 같다. 1. Glucose Isomerizing Enzyme을 분비(分泌)하는 Actinomyces과(科) 균주(菌株)를 토양(土壤)으로부터 분리(分離)하였으며 2. 본(本) 균주(菌株)가 생성(生成)하는 Glucose Isomerizing Enzyme를 Glucose에 작용(作用)시킨 결과(結果) 생성(生成)된 당(糖)은 Fructose 일종(一種)만을 생성(生成)하였으며 그외(外)의 당(糖)의 부생물(副生物)은 검출(檢出)되지 아니 하였다.

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