Korean Journal of Veterinary Research (대한수의학회지)
- Volume 8 Issue 2
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- Pages.125-146
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- 1968
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- 2466-1384(pISSN)
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- 2466-1392(eISSN)
Studies on the Biological Assay of Black leg Vaccine and Antiserum
우기종저(牛氣腫疽)에 대한 예방약(豫防藥)과 항혈청(抗血淸)의 검정(檢定)을 위한 연구(硏究)
- Kim, Dong Sung (Veterinary Research Laboratory, Office of Rural Development)
- 김동성 (농촌진흥청 가축위생연구소)
- Published : 1968.12.22
Abstract
Throughout the studies the following experimental results were summarized. 1. It was impossible to infect and kill the mice, weighing 10 to 12 gm, by inoculating 0.2ml of virulent Cl. chauvoei, diluted 1 to 10 with physiological saline, via subcutaneous, intramuscular, intraperitoneal or intraveonus, route. 2. The mice which were inoculated in brain with 0.03ml of Cl. chauvoei diluted 1 : 5120 with physiological saline were resulted in all death after infection, but not in case of attenuated strain even in dilution of one to five. 3. Virulent Cl. chauvoei were diluted with each of those of whole blood, erythrocytes and serum of horse, calf, swine, sheep, rabbit, guinea pig, chicken and duck, human plasma and 2% CaCl solution, and inoculated subcutaneously 0.25 to 0.5ml in mice, weighing 12 to 15gm. It was resulted in significant increase in virulence as comparing with the case of physiological saline solution except when horse and pig sera were used. Such a phenomena were not seen in attenuated strain. 4. Virulence of virulent Cl. Chauvoei could be increased significantly in rat, as the procedures used in mice, by suspending in whole blood, erythrocytes, serum, or plasma of various animals, or 2%
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