Methyleue blue가 X線에 照射된 흰쥐의 肝臟 glycogen, 血糖 및 血液內 電解質에 미치는 影響

Influence of Methylene Blue on Liver Glycogen, Blood Glucose, and Electrolytes in Serum of Rats Exposed to X-rays

成熱한 albino 雄性 흰쥐를 對照群과 實驗群으로 나누어 對照群에는 0.9% 生理食鹽水를, 實驗群에는 methylene blue (40mg/kg, 食鹽水에 溶解, pH 7.0)를 各各 皮下注射處理하였다. 30 分後에 兩群共히 每分當 33r 으로 總線量 300r 의 X-線을 一時 全身照射하였다. 照射後 24日間에 걸쳐 肝臟 glycogen 含量(Carroll et al. 方法), 血糖含量(Folin and Wu 方法) 및 K 와 Na 含量(flame photometer)과 Cl 含量(automatic chloride titrator)을 各各 測定하여 methylene blue의 放射線에 對한 防禦　果를 硏究調査하였다. 一般的으로 肝臟 glycogen 含量은 兩群共히 X-線照射直後 若干 減少하나 1 日區에서부터 急激한 增加를 나타내고 있으며 特히 實驗群인 methylene blue 處理群에서는 對照群인 生理食鹽水群에 比하여 增加의 遲滯性을 나타내었다. 血糖含量에 對해서는 兩群모두 X-線 照射直後 若干 減少하는 傾向을 보였으나 時日經過에 따라서 서서히 增加하며 特히 實驗根인 methylene blue 處理群은 對照群인 生理食鹽水에 比하여 增加에 遲滯性을 나타내었다. 또한 血液內 電解質含量에 있어서 K, Na 및 Cl 含量은 一般的으로 兩群모두 時日經過에 따라 큰 變化는 없었으나 特히 K와 Na의 含量은 初期狀態에서 若干의 變化가 있었고 Cl 에서는 別變化를 觀察할 수 없는것으로보아 methylene blue가 血液內 電解質含量에는 別影響이 없었다. 以上의 結果로 미루어 보아 炭水化物代謝에 있어서 methylene blue가 放射線에 對한 防禦　果가 있는 것으로 思料된다.

Male rats of the Albino strain received methylene blue in the dose of 40mg/kg by subcutaneous injection and were subjected to total body X-irradiation, 300 roentgen, at 30 minutes after the injection. The protective effect of methylene blue against the single total body X-irradiation was studied for 24 days after X-irradiation with regard to the levels of liver glycogen, blood glucose, and electrolytes in serum. 1. Total body X-irradiation generally casued an increase in the levels of the liver glycogen and blood glucose in both methylene blue treated and control group during the entire experiment. 2. Methylene blue has been shown to delay slightly the increase of the levels of the liver glycogen and blood glucose when comparing with both groups which were given methylene blue and control saline injection before irradiation in the rats. 3. The delay in the increase in the levels of liver glycogen, in experimental group injected with methylene blue, significantly came in two phases. The first phase appeared at there days after the exposure, the second followed at eighth day. It appeared that the recovery phase was at nineteenth day. 4. During the experimental days the levels of the blood glucose increased generally, methylene blue, however, caused delay in two phases; the first at fifth day, the second at eighteenth day after the exposure to X-rays. 5. In electrolytes, there was not a significant difference. The levels of chloride were, however, slightly decreased in both groups, levels of potassium appeared different in two phases at first day and twelfth day, and the levels of sodium appeared to show irregular changes at the same levels, but there was no significant difference. 6. It may be considered that methylene blue greatly reduces the sensitivity of rats to X-rays, provided that methylene blue is given before the exposure.