The Korean Journal of Pharmacology (대한약리학회지)
- Volume 2 Issue 1 Serial No. 2
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- Pages.49-69
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- 1966
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- 0377-9459(pISSN)
Studies on the Pharmacodynamic Action of Methemoglobin
Methemoglobind의 약력학적(藥力學的) 작용(作用)에 관(關)한 연구(硏究)
- Kim, Kwang-Yun (Department of Pharmacology, College of Medicine, Pusan National University)
- 김광연 (부산대학교 의과대학 약리학교실)
- Published : 1966.05.01
Abstract
For the purpose of stydying the pharmacodynamic action of methemoglobin, the author made the following experiments: 1. Preparation of hemoglobin and methemoglobin solutions: Red cell suspension from rabbit blood was hemolysed with distilled water and then divided into two portions. One portion was dialysed through cellophane paper and made isotonic with the proper amount of sodium chloride. The second portion was treated with sodium nitrite to convert hemoglobin to methemoglobin, dialysed through cellophane paper and made isotonic. 2. The concentration of methemoglobin in solution, plasma and urine was determined by Horecker and Brackette's method, and that of hemoglobin by the cyanmethemoglobin method. 3. The concentration of methemoglobin and hemoglobin in the plasma and urine of rabbits was measured at several intervals of time after infusion of the above samples. 4. The blood pressure and respiration of rabbits were recorded on a kymograph, and the effects of the samples on them were observed. 5. The effects of the samples on the movements of the in-situ heart and the isolated intestine of rabbits were studied. 6. The kidneys of rabbits were excised 4 to 5 hours after injection of the samples, and histopathological examinations were made. These experiments revealed the following results: 1. When methemoglobin solution was allowed to stand in room air, there was no decrease in the concentration of methemoglobin. 2. When methemoglobin solution was mixed with whole blood and incubated at
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