Studies on 5'-Phosphodiesterase produced by microoganisms - Part. I. On the 5'-Phosphodiesterase of Penicillium sclerotiorum -

미생물(微生物)에 의(依)한 5'-Phosphodiesterase생산(生産)에 관(關)한 연구(硏究) - 제1보(第一報) penicllium sclerotiornm의 5'-Phosphodiesterase에 대(對)하여 -

(1) The 30 strains of Penicillia and the 52 strains of Aspergilli have been screened for their producibility of 5'-Phosphodiesterase, and Penicillium sclerotiorum 7321, Penicillium sp M-11 and Penicillium citrinum UV-mutant 2032-72 were selected as having high 5‘-Phosphodiesterase activity. (2) Using the wheat bran medium the 5‘-Phosphodiesterase production was reached at maximum state by the plate culture for 10 days at $30^{\circ}C$ (3) The optimum conditions of the 5'-Phosphodiesterase activity of Penicillium sclerotiorum 7321 and Penicillium sp M-11 were pH 4.0 at $62.5^{\circ}C$, while the optimum condition of the 5'-Phosphodiesterase activity of Penicillium citrinum UV-mutant 2032-72 was pH 5.0 at $50^{\circ}C$.

Penicillium균(菌) 30주(株) Aspergillus균(菌) 52주(株)의 5'-Phosphodiesterase activity를 screen하여 강력(强力)한 Penicillium sclerotiorum 6321, Penicillium SP M-11 그리고 Penicillium citrinum UV-mutant 2032-72 선발(選拔)하였다. (2) 밀기울 배지(培地)호 $30^{\circ}C$ 10일(日)의 정치배양(靜置培養)에서 5‘-Phosphodiesterase생산(生産)이 강(强)했다. (2) Penicillium sclerotiorum 7321가 Penicillium SP M-11의 5‘-Phosphodiesterase 최적조건(最適條件)이 pH 4.0, $62.^{\circ}C$이고 Penicillium citrinum UV-mutant 2032-72 의 5'-Phosphodiesterase 최적조건(最適條件)은 PH5.0, $50^{\circ}C$이다.