미량확산분석법에 의한 청산의 정량법 (제1보) 청산배당체(행인)중의 청산의 정량

Quantitative Analysis of Prussic Acid by Micro-Diffussion Analysis. (I) Determination of Prussic Acid in HCN-Glucoside of Armeniaca.

A new quantitiative analytical method of prussic acid by "Microdiffusion analysis" was studied. HCN-Glucoside of ARMENIACA was hydrolysed with KOH in out-room of unit, and then concentrated sulfuric acid was poured in order to liberate the HCN gas. The liberated gas was absorbed into nickel sulfate solution of inner room of unit quantitatively. The excess of nickel sulfate was determined by EDTA Reagent using MX-indicator. By this method, the following results were obtained: (1) It was needed more than 4 hours, in order to hydrolyse completely at $50^{\circ}C$, but could be shortened to 3 hrs. at $^60{\circ}C$. (2) It was completely absorbed into nickel sulfate solution after 30min.