Study on the Paper Chromatography. Ⅰ. Capillary Ascending of Liguids

Paper Chromatography 에 關한 硏究 (第 1 報) 毛細管 上昇에 關하여 (基一)

The relation between the height of ascending solvents and its velocity were studied with strips of Toyo No. 50 filter paper. The filter paper strip of 3cm width and cat 30 cm length is hooked down or fixed between two sheets of glass plates inside a cylinder containing the solvent, in which the lower end of the strip is dipped. As the solvents, acetone, hydrochloric acid, water or mixtures of these are used. For short time intervals, Ostwald's folmula $h = Kt^n$ was found to be most suitable to express the relation between the height of the solvent front, h, and time, t. For longer time intervals, results will be discussed in the next paper. The mean values of the constant m in the above formula for pure acetone and pure water were 0.44 and 0.485, respectively, and that of K were 0.05 and 2.4, respectively. The time interval whose the above formula applies, for each solvent was 2 hours for the former, and a half hour for the later, respectively. The movement of solutes, such as $Cu^{++}$ and $Fe^{++}$ showed that the Rf values of solutes become constant values after a long periods; e.g., ca. 6 hours with 10N-HCl-Acetone (1 : 9) solvent.