Acknowledgement
This work was supported by the Basic Science Research Program of the National Research Foundation of Korea (NRF) and funded by the Ministry of Education (NRF-2018R1A6A1A03024862 and NRF-2022R1 I1A3055428).
Proceedings of the Plant Resources Society of Korea Conference (한국자원식물학회:학술대회논문집)
- 2023.04a
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- Pages.45-45
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- 2023
Induction of Autophagy by Rosa acicularis Leaves Extracts in RAW264.7 Cells
- Jeong Won Choi (Department of Forest Science, Andong National University) ;
- Hyeok Jin Choi (Department of Forest Science, Andong National University) ;
- Gwang Hyeon Ryu (Department of Forest Science, Andong National University) ;
- Seung Woo Im (Department of Forest Science, Andong National University) ;
- Jae Won Lee (Department of Forest Science, Andong National University) ;
- Jin Boo Jeong (Department of Forest Science, Andong National University)
- Published : 2023.04.27
Abstract
Autophagy contributes to enhancing the immune system (innate and adaptive immune system) against foreign pathogens. Autophagy of macrophages is used as a major indicator for developing vaccine adjuvants to increase the adaptive immune response. In this study, RAL increased the production of immunostimulatory mediators and phagocytotic activity in RAW264.7 cells. RAL increased p62/SQSTM1 expression. Inhibition of TLR4, JNK, and PI3K/AKT blocked RAL-mediated increase of p62/SQSTM1. RAL activated JNK and PI3K/AKT signaling. RAL-mediated activation of JNK and PI3K/AKT signaling was reversed by TLR4 inhibition. Taken together, it is believed that RAL-mediated autophagy may be dependent on activating via TLR4-dependent activation of JNK and PI3K/AKT signaling in macrophages.
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