카사바 액아배양 시 배지조성이 기내 식물체 발근에 미치는 영향

Effect of Medium Composition on in vitro Plant Root Regeneration from Axillary Buds of Cassava (Manihot esculenta Crantz)

  • 권영희 (충청북도농업기술원 원예연구과) ;
  • 최원일 (충청북도농업기술원 원예연구과) ;
  • 김희규 (충청북도농업기술원 원예연구과) ;
  • 김경옥 (충청북도농업기술원 원예연구과) ;
  • 김주형 (충청북도농업기술원 원예연구과) ;
  • 송용섭 (충청북도농업기술원 원예연구과)
  • Young Hee Kwon (Horticultural Research Division, Chungcheongbuk-do Agricultural Research and Extension Services) ;
  • Won IL Choi (Horticultural Research Division, Chungcheongbuk-do Agricultural Research and Extension Services) ;
  • Hee Kyu Kim (Horticultural Research Division, Chungcheongbuk-do Agricultural Research and Extension Services) ;
  • Kyung Ok Kim (Horticultural Research Division, Chungcheongbuk-do Agricultural Research and Extension Services) ;
  • Ju Hyoung Kim (Horticultural Research Division, Chungcheongbuk-do Agricultural Research and Extension Services) ;
  • Yong Sup Song (Horticultural Research Division, Chungcheongbuk-do Agricultural Research and Extension Services)
  • 발행 : 2021.04.29

초록

The Cassava (Manihot esculenta Crantz) is one of the major food crops in the tropical or subtropical regions. Recently, clean planting materials of improved cassava cultivars are in high demand. Problems in the propagation of cassava are virus vulnerable and low rates of seed germination. Thus, the study was undertaken to develop an efficient in vitro mass propagation protocol of Manihot esculenta Crantz. So we tried to optimize protocols for mass production from axillary buds of Cassava. Young and actively growing stem segments were excised from adult plants of cassava. Samples were cut into a 3~4 cm nodal segments with axillary buds, and cultivated in the different medium supplemented with various plant growth regulators for 4 weeks. For shoot multiplication, axillary buds approximately 1 cm in length were taken from in vitro derived shoots and subcultured. After 4~6 weeks, the shoot generation rate showed 55.6%. The shoot number and its length was 1.0/explant and 2.3 cm in the most favorable medium composition. The auxin β-indolebutyric acid(IBA) 0~2.0 mg/L was proved to be effective on root development. Plantlets with fibrous roots easily generated tuberous roots in vitro. The tuberous roots were induced only when both kinetin and IBA were used in combination. after 8 weeks, the root generation rate showed 100%. The root number and its length was 17.2/explant and 2.2 cm in the most promising medium composition. Our experiments confirmed that in vitro growth and multiplication of plantlets could depend on its reaction to the different medium composition, and this micropropagation techniques could be a useful system for healthy and vigorous plant production.

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