Proceedings of the Plant Resources Society of Korea Conference (한국자원식물학회:학술대회논문집)

The Plant Resources Society of Korea (한국자원식물학회)
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Development of PCR based Genetic Marker for Discrimination of Manchurian Clematis, Clematis terniflora var. mandshurica and Three-leaf clematis, Clematis apiifolia

으아리와 사위질빵 판별을 위한 PCR 기반의 마커 개발

  • Dae-Ju Oh (Biodiversity Research Institute, Jeju Technopark) ;
  • Eun Bi Jang (Biodiversity Research Institute, Jeju Technopark) ;
  • Jong-Du Lee (Biodiversity Research Institute, Jeju Technopark) ;
  • Hyejin Hyeon (Biodiversity Research Institute, Jeju Technopark) ;
  • Yong-Hwan Jung (Biodiversity Research Institute, Jeju Technopark)
  • 오대주 ((재)제주테크노파크 생물종다양성연구소) ;
  • 장은비 ((재)제주테크노파크 생물종다양성연구소) ;
  • 이종두 ((재)제주테크노파크 생물종다양성연구소) ;
  • 현혜진 ((재)제주테크노파크 생물종다양성연구소) ;
  • 정용환 ((재)제주테크노파크 생물종다양성연구소)
  • Published : 2021.04.29
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Abstract

To distinguish manchurian clematis, Clematis terniflora var. mandshurica and three-leaf clematis, C. apiifolia, we collected 9 nuclear ITS sequences and two sequences of trnQ-trnH intergenic spacer and trnH region in GenBank database. Those sequences were aligned to find differences between those of C. terniflora var. mandshurica and C. apiifolia. Two primer pairs were newly designed base on the differences between two species and conducted multiplex PCR. The size of amplified fragments using generated primers were 380 base pairs (only three-leaf clematis) and 189 base pairs (both species). This genetic marker based on PCR is useful to discrimination of C. terniflora var. mandshurica and C. apiifolia

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