한국자원식물학회:학술대회논문집 (Proceedings of the Plant Resources Society of Korea Conference)
- 한국자원식물학회 2020년도 추계국제학술대회
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- Pages.3-13
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- 2020
천연물 유래 물질이 감염성 질환에 미치는 영향과 효능
Effects and Efficacy of Natural Product on Infectious Diseases of pseudomonas aeruginosa
- 박지원 (한국생명공학연구원)
- Ji-Won Park (Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology)
- 발행 : 2020.12.11
초록
Pseudomonas aeruginosa is a ubiquitous gram-negative bacterium causing serious infections. The P. aeruginosa T3SS is a syringe-like apparatus on the bacterial surface, with 4 effector toxins: ExoS, ExoT, ExoY, and ExoU. Here, we investigated the effect of ExoS and ExoT of the T3SS of P. aeruginosa K strain (PAK). The type three secretion system (T3SS) is a major virulence system of Pseudomonas aeruginosa (P. aeruginosa). The effector protein Exotoxin S (ExoS) produced by P. aeruginosa is secreted into the host cells via the T3SS. For the purpose of screening the inhibitors with regard to ExoS secretion, we developed the sandwich-type enzyme-linked immunosorbent assay (ELISA) system. PAK clinical strains induce proinflammatory cytokine production through the T3SS, and this involves NF-κB activation in pneumonia mouse models. We tried to confirm the role of the NF-κB transcription factor in ExoS- and ExoT-induced pneumonia mouse models. pro-inflammatory cytokines induction in response to ExoS and ExoT infection relied on NF-κB activation. Our findings highlight the roles of natural poduct in inhibiting proinflammatory cytokine expression during ExoS and ExoT exposure in PAK infections, paving the way for a novel therapeutic approach for the treatment of pulmonary infections.